Acrosome
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The acrosome is an organelle that develops over the anterior half of the head in the spermatozoa (sperm cell) of many animals. It is a cap-like structure derived from the Golgi apparatus. Acrosome formation is completed during testicular maturation. In Eutherian mammals the acrosome contains digestive enzymes (including hyaluronidase and acrosin). [1] These enzymes break down the outer membrane of the ovum called the zona pellucida, allowing the haploid nuclei in the sperm to join with the haploid nucleus found in the ova.
This shedding of the acrosome or acrosome reaction can be stimulated in vitro by substances a sperm cell may encounter naturally such as progesterone or follicular fluid, as well as the more commonly used calcium ionophore A23187. This can be done to serve as a positive control when assessing the acrosome reaction of a sperm sample by flow cytometry [2] or fluoresence microscopy. This is usually done after staining with a fluoresceinated lectin such as FITC-PNA, FITC-PSA, FITC-ConA, or fluoresceinated antibody such as FITC-CD46.[3]
[edit] References
- ^ "acrosome definition - Dictionary - MSN Encarta". http://encarta.msn.com/encnet/features/dictionary/DictionaryResults.aspx?refid=1861695331. Retrieved on 2007-08-15.
- ^ Miyazaki et al. Archives of Andrology 25:243-251 (1990)
- ^ Carver-Ward et al. Journal of Assisted Reproduction and Genetics, Vol. 14, no. 2, 1997
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