File:DNA origami rotaxanes.jpg
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| Description | (a) A rotaxane is formed from an open ring (R1) with a flexible hinge and a dumbbell-shaped DNA origami structure (D1), which were prepared separately. The hinge of the ring consists of a series of strand crossovers into which additional thymines are inserted to provide higher flexibility. Ring and axis subunits are first connected and positioned with respect to each other using 18 nucleotide long, complementary sticky ends 33 nm away from the centre of the axis (blue regions). The ring is then closed around the dumbbell axis using closing strands (red), followed by the addition of release strands that separate dumbbell from ring via toehold-mediated strand displacement. (b) 3D models and corresponding averaged TEM images of the ring and dumbbell structure. Also shown are exemplary single-particle images. (c) TEM images of the completely assembled rotaxanes (R1D1). (d) 3D models, averaged and single-particle TEM images of R2 and D2, subunits of an alternative rotaxane design containing bent structural elements. The TEM images of the ring structure correspond to the closed (top) and open (bottom) configurations. (e) 3D representation and TEM images of the fully assembled R2D2 rotaxane. Origami models are generated using CanDo. Scale bar, 50 nm. |
| Date | |
| Source | http://www.nature.com/articles/ncomms12414 |
| Author | Jonathan List, Elisabeth Falgenhauer, Enzo Kopperger, Günther Pardatscher & Friedrich C. Simmel |
| Permission (Reusing this file) |
  This file is licensed under the Creative Commons Attribution 4.0 International license.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 02:18, 17 March 2017 | | 2,260 × 1,440 (278 KB) | Materialscientist | from PDF |
| 02:14, 17 March 2017 |  | 926 × 595 (199 KB) | Materialscientist | {{Information |Description=(a) A rotaxane is formed from an open ring (R1) with a flexible hinge and a dumbbell-shaped DNA origami structure (D1), which were prepared separately. The hinge of the ring consists of a series of strand crossovers into whic... |
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