File:Yoctoreactor library assembly wiki3.JPG
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Summary
| Description |
English: Figure 6. Stepwise assembly of a DEL library using YoctoReactor technology. A 3-way reactor is shown here. (a) Position 1 (P1) and P2 BB are brought into close proximity and undergo a chemical reaction in the presence of a helper oligonucleotide in P3. (b) The structure is purified by polyacrylamide gel electrophoresis (PAGE), the P1 and P2 DNA is ligated and the P2 linker is cleaved. (c) P3 BB is annealed to the P1-P2 ligation product from step b, and a chemical reaction between P2 and P3 BBs takes place. (d) The reaction product is purified by PAGE, the DNA is ligated and P3 linker is cleaved yielding a compound (OOO) covalently attached to the folded yR. (e) The yR is dismantled by primer extension yielding a double-stranded disply product exposing the reaction product for selection and molecular evolution. |
| Date | |
| Source | Own work |
| Author | Viptrh |
Licensing
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I, the copyright holder of this work, release this work into the public domain. This applies worldwide. In some countries this may not be legally possible; if so: I grant anyone the right to use this work for any purpose, without any conditions, unless such conditions are required by law. |
File history
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 08:38, 25 May 2010 | | 288 × 720 (22 KB) | Viptrh | improved small details of image |
| 08:36, 25 May 2010 |  | 288 × 720 (22 KB) | Viptrh | improved small details of image | |
| 08:33, 25 May 2010 |  | 288 × 720 (22 KB) | Viptrh | Improved small details of image | |
| 08:03, 25 May 2010 |  | 288 × 720 (22 KB) | Viptrh | Improved small details of image. | |
| 08:00, 25 May 2010 |  | 288 × 720 (22 KB) | Viptrh | Improved small details of image. | |
| 07:56, 25 May 2010 |  | 288 × 720 (22 KB) | Viptrh | powerpoint image improved. Small details. | |
| 11:45, 21 May 2010 |  | 288 × 720 (21 KB) | Viptrh | {{Information |Description={{en|1=Figure 6. Stepwise assembly of a DEL library using YoctoReactor technology. A 3-way reactor is shown here. (a) Position 1 (P1) and P2 BB are brought into close proximity and undergo a chemical reaction in the presence of |
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