AaaI

AaaI is a Type II restriction enzyme found in Acetobacter aceti ss aceti.^[1] Its prototype is XmaIII. It is in the subtype category 'P', meaning that it has symmetric target and cleavage sites.

Its recognition sequence is 5' CGGCG and 3' GCCGGC and its cut is 5' ---C GGCCG--- 3' and 3' ---GCCGG C--- 5'.

Its isoschizomers are BseX3I, BstZI, EagI, EclXI, Eco52I, SenPT16I, XmaIII, meaning that they have the same recognition sequences, but have been isolated from different bacteria, so they likely have different reaction conditions, making them different.

References[edit]

^ "RJR REBASE Enz 1 - AaaI". rebase.neb.com. Retrieved 2019-06-15.

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[1] "RJR REBASE Enz 1 - AaaI". rebase.neb.com. Retrieved 2019-06-15.

[1]

v t e Restriction modification system: restriction enzyme
Basic Concept	Isoschizomer Neoschizomer Isocaudomer
Recognition sequence 4bp	TaqI Sau3A AluI* HaeIII* DpnII NlaIII HpaII
Recognition sequence 5bp	EcoRII FokI
Recognition sequence 6bp	AaaI BglII PovII* EcoRV* EcoRI BamHI HindIII XbaI XhoI SacI PstI NdeI
Recognition sequence 8bp	NotI
Lists	A Ba–Bc Bd–Bp Bsa–Bso Bsp–Bss Bst–Bv C-D E-F G-K L-N O-R S T-Z
* means cleavage produces blunt ends