C10orf35

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The blue highlighted line shows the location of the c10orf35 gene on Chromosome 10.

Chromosome 10 open reading frame 35 (c10orf35) is a gene that in humans, encodes for a protein-binding,[1] transmembrane[2] protein. The protein contains the domain of unknown function 4605 (DUF4605)[1] which belongs to the protein family pfam15378.[3] This gene is located at locus 10q22.1.[1]

General characteristics[edit]

The physical properties of the c10orf35 protein were analyzed, and the molecular weight was predicted to be 13.2 kdal,[4] and the isoelectric point was predicted to be 11.5.[4] The properties of DUF4605 were also analyzed, and the molecular weight was predicted to be 6.0 kdal.[4] The isoelectric point was predicted to be 6.9,[4] which is significantly less basic than the full protein.

Properties and structure[edit]

This image gives a predicted image of the main secondary structure features, conserved regions, and the post-translational modification site.

This protein includes a highly conserved region between amino acids 1 and 19 discovered by MSA.[5] DUF 4605 is located between amino acids 62 and 92.[1] The secondary structure of the c10orf35 protein was predicted to consist of a β-sheet between amino acids 3 and 5,[6] an α-helix between 61 and 72,[6] and a transmembrane α-helix between 92 and 112.[7] The serine in position 62 can be phosphorylated to form a phosphoserine group.[7]

Homology[edit]

Paralogs[edit]

The protein of interest has a similar, paralogous domain found in c4orf32,[8] which is conserved in mammals, birds, very few reptiles, and one amphibian.[9]

Orthologs[edit]

The protein coded for by c10orf35 has many orthologs found mainly in mammals, reptiles, few amphibians, one fish, and one invertebrate.[10] Based on a multiple sequence alignment between representative orthologs, DUF 4605 and a second, highly conserved region were confirmed.[5]

Clinical significance[edit]

Expression[edit]

This image shows the expression of the c10orf35 protein in various human tissues.[11]

The c10orf35 protein has expression over the 75th percentile in the brain, spinal cord, and male reproductive organs.[12] Within the male reproductive system, the RNA is found within the testis and prostate, while the protein is expressed in the epididymis.[13] In the brain, the RNA is found in the cerebral cortex, while the protein is expressed ubiquitously throughout the brain.[13]

Transcription regulation[edit]

Protein interactions[edit]

The c10orf35 protein has been experimentally shown by Affinity Capture-MS to interact with 7 other proteins[1] as shown below.

Interacting Protein Symbol Definition
ATP1B3 ATPase, Na+/K+ Transporting, Beta 3 Polypeptide
CD3E CD3e molecule, epsilon (CD3-TCR complex)
GABRE Gamma-aminobutyric acid (GABA) A receptor, epsilon
HTR3C 5-hydroxytryptamine (serotonin) receptor 3C, ionotropic
LPAR1 Lysophosphatidic acid receptor 1
SGCD Sarcoglycan, delta (35kDa dystrophin-associated glycoprotein)
ZACN Zinc activated ligand-gated ion channel

References[edit]

  1. ^ a b c d e "c10orf35". National Center For Biotechnology Information (NCBI) Gene.
  2. ^ "SOSUI". Classification and Secondary Structure Prediction of Membrane Proteins. Retrieved 6 May 2016.
  3. ^ "Conserved Protein Domain Family DUF4605". National Center For Biotechnology Information (NCBI) Structure. Retrieved 6 May 2016.
  4. ^ a b c d "Compute pI/Mw". ExPASy Bioinformatics Research Portal. Retrieved 6 May 2016.
  5. ^ a b "CLUSTALW Alignment Tool". San Diego Supercomputer Center (SDSC). Retrieved 6 May 2016.
  6. ^ a b "A Protein Secondary Structure Prediction Server". JPred. Retrieved 9 May 2016.
  7. ^ a b "c10orf35". UniProt. Retrieved 9 May 2016.
  8. ^ "c4orf32". National Center For Biotechnology Information (NCBI) Gene. Retrieved 6 May 2016.
  9. ^ "Standard Nucleotide BLASTN queried for c4orf32". National Center For Biotechnology Information (NCBI) Basic Local Alignment Searth Tool (BLAST). Retrieved 6 May 2016.
  10. ^ "Standard Nucleotide BLAST queried for c10orf35". National Center For Biotechnology Information (NCBI) Basic Local Alignment Search Tool (BLAST). Retrieved 6 May 2016.
  11. ^ "Gene Expression Omnibus (GEO) Profile For GDS3834". National Center For Biotechnology Information (NCBI). Retrieved 9 May 2016.
  12. ^ "Gene Expression Omnibus (GEO) Profile". National Center For Biotechnology Information. Retrieved 6 May 2016.
  13. ^ a b "c10orf35". The Human Protein Atlas. Retrieved 6 May 2016.
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