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Collagen hybridizing peptide

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Schematic of a CHP strand (labeled with an "X" tag) hybridizing to denatured collagen chains and forming a collagen triple helix. During disease progression, tissue development, or ageing, collagen can be extensively degraded by collagenolytic proteases, causing its triple helix to unfold at the physiological temperature due to reduced thermal stability. X may represent a biotin or fluorescent tag.

A collagen hybridizing peptide (CHP) is a synthetic peptide sequence with typically 6 to 10 repeating units of the Gly-Xaa-Yaa amino acid triplet, which mimics the hallmark sequence of natural collagens.[1][2] A CHP peptide usually possesses a high content of proline and hydroxyproline in the Xaa and Yaa positions, which confers it a strong propensity to form the collagen's unique triple helix conformation.[1][3] In the single-stranded (monomeric) status, the peptide can recognize denatured collagen strands in tissues by forming a hybridized triple helix with the collagen strands.[2] This occurs via the triple helical chain assembly and inter-chain hydrogen bonding, in a manner similar to primers binding to melted DNA strands during PCR.[4] The binding does not depend on a specific sequence or epitope on collagen, enabling CHPs to target denatured collagen chains of different types.[5][6]

Collagen, CHP, CMP, and CLP

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Schematic showing relationship between the CMP and CHP. Triple helical CMPs can be heated (above a defined temperature) to dissociate into monomeric CHPs; upon cooling, CHP strands can re-assemble into a triple helix over time.

Collagen is the main component of the extracellular matrix (ECM).[7] The collagen superfamily consists of 28 different types of collagen.[7] Although the function and hierarchical structure of these collagens may vary, they all share the defining structural feature known as the triple helix,[1] where three left handed polyproline II-type (PPII) helices assemble to form a right-handed supercoiled helical motif.[1][8] Short synthetic peptides known as collagen mimetic peptides (CMPs) or collagen-like peptides (CLPs) have played a major role in elucidating the 3D structure of the collagen triple helix, its folding kinetics, and thermal stability as small triple helical models.[3][9][10][11] CMPs, CLPs, and CHPs are all very similar in terms of their amino acid sequences but only when CMPs or CLPs are heated above their melting temperatures, do they exist in the dissociated, single-stranded state and can be considered as CHPs.[2]

Binding mechanism

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Single-stranded CHPs bind to denatured collagen chains and gelatin in a manner that is unique from other targeting mechanisms, in that they specifically recognize a unique structural motif (collagen triple helix) for folding and chain assembly, as opposed to specific epitopes binding that is seen for monoclonal antibodies (mAbs), for example.[12] Due to their unique targeting mechanism, CHPs have a high binding specificity towards denatured collagen chains but have almost no affinity for intact (triple helical) collagen.[13] CHPs can broadly target collagen chains that have been denatured by thermal,[13] chemical,[14] mechanical,[15] or enzymatic processes,[13] as well as multiple collagen types (e.g., Col I, II, IV).[5][6] Studies also showed CHPs and their fluorophore conjugates have superior stability in contact with serum.[16]

Denatured collagen as a biomarker for tissue remodelling and damage

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A fluorescence image of an axial cross section of a mouse heart at day 14 post myocardial infarction, stained with Hoechst 33342 (blue) and biotin-labeled CHP (detected with AlexaFluor647-streptavidin, red). Scale bar: 1 mm.

Controlled collagen turnover is crucial for embryonic development, organ morphogenesis, as well as tissue maintenance and repair.[17] However, changes of collagen homeostasis are associated with numerous diseases and pathological conditions. Excessive collagen degradation may be associated with cancer metastasis, skin ageing, arthritis, and osteoporosis.[17] CHPs can target tissues undergoing remodelling based on their ability to bind to degraded and unfolded collagen strands through triple helix formation. As a targeting moiety, CHPs offer great potential in histopathology, diagnostics, and drug delivery for a wide range of diseases.

Most methods for the evaluation of collagen denaturation in disease states are indirect, such as detecting matrix metalloproteinase (MMP) activity or quantifying collagen peptide fragments in urine, serum, or synovial fluid.[18][19][20] Using conventional methods for directly targeting collagen, researchers have to relied on collagen binding peptides selected by phage display,[21] derived from collagen binding proteins,[22] or antibodies raised against collagens. Unfortunately, these compounds cannot target denatured collagens which are unstructured and do not present a defined 3D epitope. In addition, antibodies that were reported to distinguish specific degraded collagen fragments can only recognize one or few collagen types.[2][23] In contrast, CHPs, in principle, can bind to all types of denatured collagens.[4][5][6]

Applications

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Tissue staining

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A fluorescence image of a sagittal section of an 18 d.p.c. mouse embryo double stained with biotinylated-CHP (detected by AlexaFluor647-streptavidin, orange) and an anti-collagen I antibody (detected by AlexaFluor555-labeled donkey anti-rabbit IgG H&L, cyan). mx, maxilla; md, mandibular bone; bp, basisphenoid bone; bo, basioccipital bone; vc, vertebral column; rb, rib; h, hipbone; d, digital bones. Scale bar: 3 mm.

Fluorophore- or biotin-labeled CHPs are used as a staining agent for detecting collagen degradation and denaturation via immunofluorescence and immunohistochemistry applications.[5] CHPs can stain frozen tissue sections, formalin-fixed paraffin embedded (FFPE) sections,[5] as well as fresh tissues.[14][15] CHP is applicable to tissue specimens from multiple species and a range of diseases, such as myocardial infarction, arthritis, nephritis, and fibrosis.[5]

In vivo imaging

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CHPs can also be labelled with near-infrared fluorophores for in vivo fluorescent imaging.[13][24]

Collagen identification

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CHPs can be used for visualizing many different types of collagen bands in SDS-PAGE gels.[6] Collagen is denatured by heating in the presence of SDS prior to loading the gel. The collagen bands are visualized through CHP-collagen hybridization when the gels are stained by fluorescently-labeled CHPs.[6]

Detecting mechanical damage to connective tissue

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Collagen offers mechanical strength in load bearing tissues in the body such as tendons, ligaments, and bone. As forces are applied to these tissues, the collagen triple helix can be damaged and unwind, and CHPs allow for molecular level detection of mechanical damage in such connective tissues.[15][25]

References

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  1. ^ a b c d Shoulders, Matthew D.; Raines, Ronald T. (2009). "Collagen structure and stability". Annual Review of Biochemistry. 78: 929–958. doi:10.1146/annurev.biochem.77.032207.120833. ISSN 1545-4509. PMC 2846778. PMID 19344236.
  2. ^ a b c d Wahyudi, Hendra; Reynolds, Amanda A.; Li, Yang; Owen, Shawn C.; Yu, S. Michael (October 2016). "Targeting collagen for diagnostic imaging and therapeutic delivery". Journal of Controlled Release. 240: 323–331. doi:10.1016/j.jconrel.2016.01.007. PMC 4936964. PMID 26773768.
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  13. ^ a b c d Li, Y.; Foss, C. A.; Summerfield, D. D.; Doyle, J. J.; Torok, C. M.; Dietz, H. C.; Pomper, M. G.; Yu, S. M. (2012-08-27). "Targeting collagen strands by photo-triggered triple-helix hybridization". Proceedings of the National Academy of Sciences. 109 (37): 14767–14772. doi:10.1073/pnas.1209721109. ISSN 0027-8424. PMC 3443117. PMID 22927373.
  14. ^ a b Hwang, Jeongmin; San, Boi Hoa; Turner, Neill J.; White, Lisa J.; Faulk, Denver M.; Badylak, Stephen F.; Li, Yang; Yu, S. Michael (April 2017). "Molecular assessment of collagen denaturation in decellularized tissues using a collagen hybridizing peptide". Acta Biomaterialia. 53: 268–278. doi:10.1016/j.actbio.2017.01.079. ISSN 1742-7061. PMC 5462463. PMID 28161576.
  15. ^ a b c Weiss, Jeffrey A.; Yu, S. Michael; Buehler, Markus J.; Reese, Shawn P.; Depalle, Baptiste; San, Boi Hoa; Qin, Zhao; Li, Yang; Zitnay, Jared L. (2017-03-22). "Molecular level detection and localization of mechanical damage in collagen enabled by collagen hybridizing peptides". Nature Communications. 8: 14913. doi:10.1038/ncomms14913. ISSN 2041-1723. PMC 5364439. PMID 28327610.
  16. ^ Bennink, Lucas L.; Smith, Daniel J.; Foss, Catherine A.; Pomper, Martin G.; Li, Yang; Yu, S. Michael (2017-05-08). "High Serum Stability of Collagen Hybridizing Peptides and Their Fluorophore Conjugates". Molecular Pharmaceutics. 14 (6): 1906–1915. doi:10.1021/acs.molpharmaceut.7b00009. ISSN 1543-8384. PMC 8063002. PMID 28445649.
  17. ^ a b Bonnans, Caroline; Chou, Jonathan; Werb, Zena (December 2014). "Remodelling the extracellular matrix in development and disease". Nature Reviews Molecular Cell Biology. 15 (12): 786–801. doi:10.1038/nrm3904. ISSN 1471-0072. PMC 4316204. PMID 25415508.
  18. ^ Nemirovskiy, O.V.; Dufield, D.R.; Sunyer, T.; Aggarwal, P.; Welsch, D.J.; Mathews, W.R. (February 2007). "Discovery and development of a type II collagen neoepitope (TIINE) biomarker for matrix metalloproteinase activity: From in vitro to in vivo". Analytical Biochemistry. 361 (1): 93–101. doi:10.1016/j.ab.2006.10.034. PMID 17187753.
  19. ^ Garvican, Elaine R.; Vaughan-Thomas, Anne; Innes, John F.; Clegg, Peter D. (July 2010). "Biomarkers of cartilage turnover. Part 1: Markers of collagen degradation and synthesis". The Veterinary Journal. 185 (1): 36–42. doi:10.1016/j.tvjl.2010.04.011. PMID 20488735.
  20. ^ Rousseau, Jean-Charles; Delmas, Pierre D (June 2007). "Biological markers in osteoarthritis". Nature Clinical Practice Rheumatology. 3 (6): 346–356. doi:10.1038/ncprheum0508. ISSN 1745-8382. PMID 17538566. S2CID 13168927.
  21. ^ Helms, Brett A.; Reulen, Sanne W. A.; Nijhuis, Sebastiaan; Graaf-Heuvelmans, Peggy T. H. M. de; Merkx, Maarten; Meijer, E. W. (2009-08-26). "High-Affinity Peptide-Based Collagen Targeting Using Synthetic Phage Mimics: From Phage Display to Dendrimer Display". Journal of the American Chemical Society. 131 (33): 11683–11685. doi:10.1021/ja902285m. ISSN 0002-7863. PMID 19642697.
  22. ^ Liang, Hui; Li, Xiaoran; Chen, Bing; Wang, Bin; Zhao, Yannan; Zhuang, Yan; Shen, He; Zhang, Zhijun; Dai, Jianwu (July 2015). "A collagen-binding EGFR single-chain Fv antibody fragment for the targeted cancer therapy". Journal of Controlled Release. 209: 101–109. doi:10.1016/j.jconrel.2015.04.029. PMID 25916496.
  23. ^ Freimark, Bruce; Clark, Derek; Pernasetti, Flavia; Nickel, Jeff; Myszka, David; Baeuerle, Patrick A.; Van Epps, Dennis (July 2007). "Targeting of humanized antibody D93 to sites of angiogenesis and tumor growth by binding to multiple epitopes on denatured collagens". Molecular Immunology. 44 (15): 3741–3750. doi:10.1016/j.molimm.2007.03.027. PMID 17507095.
  24. ^ Bennink, Lucas L.; Li, Yang; Kim, Bumjin; Shin, Ik Jae; San, Boi Hoa; Zangari, Maurizio; Yoon, Donghoon; Yu, S.Michael (November 2018). "Visualizing collagen proteolysis by peptide hybridization: From 3D cell culture to in vivo imaging". Biomaterials. 183: 67–76. doi:10.1016/j.biomaterials.2018.08.039. PMID 30149231.
  25. ^ Converse, Matthew I.; Walther, Raymond G.; Ingram, Justin T.; Li, Yang; Yu, S. Michael; Monson, Kenneth L. (2018-02-01). "Detection and characterization of molecular-level collagen damage in overstretched cerebral arteries". Acta Biomaterialia. 67: 307–318. doi:10.1016/j.actbio.2017.11.052. ISSN 1742-7061. PMC 5794621. PMID 29225149.