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Subfossil lemurs are lemurs from Madagascar that are represented by recent (subfossil) remains dating from the late Pleistocene to approximately 560 years ago. They include both living and extinct species, although the term more frequently refers to the extinct giant lemurs. The diversity of subfossil lemur communities was higher than that of present-day lemur communities, ranging as high as 20 or more species per location, compared to 10 to 12 species today. Extinct species ranged in size from slightly over 10 kg (22 lb) to roughly 200 kg (440 lb). Even the subfossil remains of living species are larger and more robust than modern specimens. The subfossil sites found around most of the island demonstrate that most giant lemurs had wide distributions and that ranges of living species have contracted significantly since the arrival of humans.

Despite their size, the giant lemurs shared many features with living lemurs, including rapid development, poor day vision, relatively small brains, and lack of male dominance. They also had many distinct traits among lemurs, including a tendency to rely on terrestrial locomotion, slow climbing, and suspension instead of leaping, as well as a greater dependence on leaf-eating and seed predation. The giant lemurs likely filled ecological niches now left vacant, particularly seed dispersal for plants with large seeds. There were three distinct families of giant lemur, including the Palaeopropithecidae (sloth lemurs), Megaladapidae (koala lemurs), and Archaeolemuridae (monkey lemurs). Two other types were more closely related and similar in appearance to living lemurs: the giant aye-aye and Pachylemur, a genus of "giant ruffed lemurs". Subfossil remains were first discovered on Madagascar in the 1860s, but giant lemur species were not formally described until the 1890s. The paleontological interest sparked by the initial discoveries resulted in an overabundance of new species names, the allocation of bones to the wrong species, and inaccurate reconstructions during the early 1900s. Discoveries waned during the mid-1900s, although paleontological work resumed in the 1980s and resulted in the discovery of new species and a new genus. The remains of the subfossil lemurs are relatively recent, with all or most species dating within the last 2,000 years. Humans first arrived on Madagascar around that time and likely played a role in the demise of the lemurs and the other megafauna that once existed on the large island. Although hunting and habitat change have been explored as the primary cause of their extinction, a mosaic of complex interactions between multiple factors is now seen as the ultimate cause of their disappearance. Yet oral traditions and recent sightings by Malagasy villagers are still reported, suggesting either lingering populations or very recent extinctions.