Talk:Illumina dye sequencing

Molecular Biology: Genetics / MCB / COMPBIO

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Wiki Education Foundation-supported course assignment[edit]

This article was the subject of a Wiki Education Foundation-supported course assignment, between 12 May 2020 and 22 June 2020. Further details are available on the course page. Student editor(s): Brizileigh. Peer reviewers: Tanner Stenlund, Bucear.

Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 22:50, 17 January 2022 (UTC)[reply]

Paired sequencing[edit]

In "Sequencing by synthesis", if the reverse strands are "washed away", how is paired-end sequencing performed? --87.138.112.29 (talk) 05:50, 1 September 2018 (UTC)[reply]

It seems that after the forward strand is sequenced, a new reverse strand is made, and then sequenced. Gah4 (talk) 22:41, 19 February 2021 (UTC)[reply]

Comparison with other methods[edit]

Comparing Illumina method with Sanger's is perhaps a bit outdated. I do not think many people use Sanger any more. I think a comparison with long reads approaches such as PacBio or Nanopore would be more meaningful. The latter would also permit a comparison between massive centralised sequencing and portable on-site one. NicGambarde (talk) 08:06, 23 June 2021 (UTC)[reply]

Clonal Amplification Section?[edit]

The "Clonal Amplification" section seems to just repeat the bridge amplification section and then discuss error detection, which should be in the "Data Analysis" section... FoldedGenes (talk) 23:12, 6 November 2023 (UTC)[reply]