User:Hagi90210/Discovery and development of nucleoside and nucleotide reverse transcriptase inhibitors

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Discovery and development of nucleoside and nucleotide reverse transcriptase inhibitors Discovery and development of nucleoside and nucleotide reverse transcriptase inhibitors (NRTIs and NtRTIs) began in the 1980s when the AIDS epidemic hit Western societies. NRTIs inhibit the reverse transcriptase (RT), an enzyme that controls the replication of the genetic material of the human immunodeficiency virus (HIV). The first NRTI was zidovudine, approved by the FDA in 1987 which was the first step towards treatment of HIV. Six NRTIs agents and one NtRTI have followed. Drug resistance viruses is an inevitable consequence of prolonged exposure of HIV-1 to anti-HIV drugs. This is a serious clinical concern and that is why further development is a necessity.

History[edit]

In the summer of 1981 the acquired immunodeficiency syndrome (AIDS) was first reported[1]. Two years later the etiological link to AIDS, the human immunodeficiency virus (HIV) was identified[2][3]. Since the identification of HIV the development of effective antiretroviral drugs and the scientific achievements in HIV research has been enormous[3]Cite error: The <ref> tag has too many names (see the help page).. Antiretroviral drugs for the treatment of HIV infections belong to six categories: Nucleoside and nucleotide reverse transcriptase inhibitors, Non-nucleoside reverse transcriptase inhibitors, protease inhibitors, entry inhibitors, co-receptor inhibitors and integrase inhibitorsCite error: The <ref> tag has too many names (see the help page).. The reverse transcriptase of HIV-1 has been the main foundation for the developement of anti-HIV drugsCite error: The <ref> tag has too many names (see the help page).. The first nucleoside reverse transcriptase inhibitor with in vitro anti-HIV activity was zidovudine[4]. Since zidovudine was approved in 1987, six nucleosides and one nucleotide reverse transcriptase inhibitor (NRTI) have been approved by FDA[4]. NRTIs approved by the FDA are zidovudine, didanosine, zalcitabine, stavudine, lamivudine, abacavir and emtricitabine and the only nucleotide reverse transcriptase inhibitor (NtRTI) approved is tenofovir (table 4)Cite error: The <ref> tag has too many names (see the help page).[4].

The HIV-1 reverse transcriptase enzyme[edit]

Function[edit]

File:Verkunarmatilokaloka.jpg
Figure 1 A: Mechanism of action of nucleoside analogues reverse transriptase inhibitors, for example zidovudine. B: Mechanism of action of the nucleotide analogue reverse transcriptase inhibitor, tenofovir

Most standard HIV drug thearapies revolve around inhibiting the reverse transcriptase enzyme (RT), an enzyme that is necessary to the HIV-1 virus and other retroviruses to complete their life cycleCite error: The <ref> tag has too many names (see the help page).. The RT enzyme serves two key functions. First, it controls the replication of the viruses genetic material via its polymerase activity. It converts the viral single-stranded RNA into an integration competent double stranded DNA. Subsequently the generated DNA is translocated into the nucleus of the host cell where it is integrated in its genome by the retroviral integrase. The other role of the RT is its ribonuclease H activity that degrades RNA only when it is in a heteroduplex with DNA[5]Cite error: The <ref> tag has too many names (see the help page)..

Structure[edit]

HIV-1 RT is an asymmetric heterodimer which is 1000 amino acid long and is composed of two subunits. The larger subunit, p66, is 560 amino acid long and it exhibits all the enzymatic activities of the RTCite error: The <ref> tag has too many names (see the help page)..The smaller subunit, called p51, is 440 amino acid long and it is considered to stabilizes the heterodimer but also it may take part in the binding of the tRNA primer. The p66 subunit has the two active sites: polymerase and ribonuclease H. The polymerase has four subdomains that have been named “fingers“, “thumb“, “connection“ and “palm“ for it has been compared to the right hand[5]Cite error: The <ref> tag has too many names (see the help page).[6].

Mechanism of action[edit]

Activation of nucleoside and nucleotide reverse transcriptase inhibitors is primarily dependent on cellular entry by passive diffusion or carrier-mediated transport. NRTIs are highly hydrophilic and have limited membrane permeability and therefore this step is very important. NRTIs are analogues of endogenous 2´-deoxy-nucleoside and nucleotide. They are inactive in their parent forms and require successive phosphorylation[4].

Nucleosides must be triphosphorylated, while nucleotides, which possess one phosphonated group, must be diphosphorylatedCite error: The <ref> tag has too many names (see the help page).. This stepwise activation process occurs inside the cell and is mediated by a coordinated series of enzymesCite error: The <ref> tag has too many names (see the help page).. The first, and often rate limiting, phosphorylation step (for nucleoside analogues) are most commonly catalyzed by deoxynucleoside kinases. Addition of the second phosphate group to nucleoside monophosphate analogues is completed by the nucleoside monophosphate kinases (NMP kinases). A variety of enzymes are able to catalyze the final phosphorylation step for NRTIs, including nucleoside diphosphate kinase (NDP kinase), phosphoglycerate kinase, pyruvate kinase and creatine kinase, resulting in formation of respective antivirally active triphosphate analogues[4]. In their respective triphosphate forms, NRTIs and the only NtRTI available compete with their corresponding endogenous deoxynucleotide triphosphate (dNTPs) for incorporation into the nascent DNA chain[4]. Unlike dNTPs substrate, NRTIs lack a 3´-hydroxyl group on the deoxyribose moiety. Once incorporated into the DNA chain, the absence of a 3´OH group, which normally forms the 5´- to 3´- phosphoester bond with the next nucleic acid, blocks further extension of the DNA by RT, and they act as chain terminatorsCite error: The <ref> tag has too many names (see the help page).Cite error: The <ref> tag has too many names (see the help page)..

Discovery and development[edit]

First step towards treatment of HIV- ziduvodine[edit]

In 1964 zidovudine (AZT) was synthesized by Horwitz at the Michigan Cancer Foundation. The 3´hydroxyl group in the deoxyribose ring of thymidine is replaced by an azido group which gives us zidovudine[7]. The lack of the 3´hydroxyl group which provides the attachment point for the viral nucleotide in the growing DNA chain during the reverse transcription makes it an obligate chain terminator. Ziduvodine is incorporated in place of thymidine and is an extremely potent inhibitor of HIV replication[8]. This compound had been prepared in 1964 as a potential anti-cancer agent but was shown to be ineffective[9]. In 1974 zidovudine was reported to have activity against retroviruses and was subsequently re-screened as an antiviral when the AIDS epidemic hit Western societies during mid 1980‘s[7][9]. However, ziduvodine is relatively toxic since it is converted into the triphosphate by the cellular enzymes and therefore it is activated in uninfected cells[8].

Further development of nucleoside analogues[edit]

Dideoxynucleosides[edit]

Table 1 Comparison of chemical structures: Dideoxyadenosine and didanosine
Dideoxyadenosine Didanosine
Chemical

structure

File:Dideoxyadenosine meed hring.jpg File:Didanosine med hring.jpg

Dideoxynucleosides are analogues of nucleoside where the sugar ring lacks both 2´ and 3´-hydroxyl groups[6]. Three years after the synthesis of zidovudine, Jerome Horwitz and his colleagues in Chicago prepared another dideoxynucleoside now known as zalcitabine (ddC)[10]. Zalcitabine is a synthetic pyrimidine nucleoside analogue, structurally related to deoxycytidine, in which the 3´-hydroxyl group of the ribose sugar moiety is substituted with hydrogen[11]. Zalcitabine was approved by the FDA for the treatment of HIV-1 in June 1992[3][12].

In 1964 dideoxyadenosine, the corresponding adenosine analogue of zalcitabine was synthesised. Dideoxyadenosine caused kidney damage so didanosine was prepared from dideoxyadenosine by enzymatic oxidation. It was found to be active against HIV without causing kidney damage[10]. Didanosine was approved by the FDA for the treatment of HIV-1 in October 1991[12]. Zalcitabine and didanosine are both obligate chain terminators, that have been developed for anti-HIV treatment. Unfortunately, both drugs lack selectivity and therefore cause side-effects[8]. Further modification of the dideoxy framework led to the development of 2´,3´-didehydro-3´-deoxythymidine (stavudine, d4T). Activity of stavudine was shown to be similar to that of zidovudine, although their phosphorylation patterns differ; the affinity for zidovudine to thymidine kinase (the enzyme responsible for the first phosphorylation) is similar to that of thymidine,whereas the affinity for stavudine is 700-fold weaker[6].

Table 2 Comparison of chemical structures : Zalcitabine and lamivudine
Zalcitabine Lamivudine
Chemical

structure

2',3'‐dideoxy‐3'‐thiacytidine (lamivudine, 3TC) was discovered by Bernard Belleau. The history of lamivudine can be traced back to the mid‐seventies while Bernard Belleau was investigating sugar derivatives. Lamivudine was developed as the sulfur analogue of zalcitabine[10]. It was initially synthesized as a racemic mixture (BCH-189) and analysis showed that both positive and negative enantiomers of BCH-189 had in vitro activity against HIV. Lamivudine which is the negative enantiomer of 2',3'‐dideoxy‐3'‐thiacytidine and is a pyrimidine nucleoside analog. The 3' carbon of the ribose ring of 2'-deoxycytidine has been replaced by a sulfur atom because it had greater anti-HIV activity and is less toxic than the positive enantiomer[10][13][14].

Next in line was 2',3'‐dideoxy‐5-fluoro-3'‐thiacytidine (Emtricitabine, FTC) which is a structural homologue of lamivudine. The structural difference is the 5-fluoro-modification of the base moiety of lamivudine. It is similar in many ways to lamivudine and is active agains both HIV-1 and hepatitis B virus (HBV)[14][15].

Carbocyclic nucleoside[edit]

Carbocyclic analogues of dideoxyadenosine were investigated for their anti-HIV activity. Minimal activity was first observed. Many nucleoside analogues were prepared and examined but only one had significant activity and satisfied the requirements for clinical use. That was 2´,3´-didehydro analogue of dideoxyadenosine. Insertion of a cyclopropyl group on its 6-amino nitrogen of the adenine ring increased lipophilicity and thus enhanced brain penetration. The resulting compound is known as abacavir[10]. Abacavir was approved by the FDA for use in therapy of HIV-1 infections in December 1998[13].


Table 3 Comparison of chemical structures: Dideoxyadenosine to didanosine
Dideoxyadenosine Didanosine Abacavir
Chemical structure File:Dideoxyadenosine meed hring.jpg File:Didanosine med hring.jpg

Acyclic nucleotide – the only approved NtRTI[edit]

Nucleotide analogues require only two phosphorlylation steps whereas nucleoside analogues require three steps. Reduction in the phosphorylation requirement may allow more rapid and complete conversion of drugs to their active metabolites. Such considerations have led to the development of phosphonate nucleotide analogues such as tenofovir. Tenofovir disoproxil fumarate (Tenofovir DF) is the prodrug of tenofovir. Tenofovir is an acyclic adenosine derivative. The acyclic nature of the compound and its phosphonate moiety are unique structural features among the approved NRTIs[14]. Tenofovir DF is hydrolyzed enzymatically to tenofovir which exhibits anti-HIV activity[16][17]. It was developed by the synthesis and broad-spectrum antiviral activity of 2,3-dihydroxypropyladenine[17]. Tenofovir DF was the first nucleotide reverse transcriptase inhibitor approved by the FDA for the treatment of HIV-1 infection in October 2001[12][16].

Resistance[edit]

Currently, appearance of drug resistant viruses is an inevitable consequence of prolonged exposure of HIV-1 to antiretroviral therapy. Drug resistance is a serious clinical concern in treatment of viral infection, and it is a particularly difficult problem in treatment of the HIV virusCite error: The <ref> tag has too many names (see the help page).. Resistance mutations are known for all approved NRTIsCite error: The <ref> tag has too many names (see the help page)..

Nucleotide analogues Nucleoside analogues
Analoug style="text-align: center; border-right: 2px solid black;"
Purine analogues
colspan="5" style="text-align: center; border-right: 2px solid black;"
Pyrimidine analogues
Purine

analogues

Nucleoside style="text-align: center; border-right: 2px solid

black;"|
Adenosine

 [[Image:dT

chemical structure.png|87px|Chemical structure of thymidine]]
Deoxythymidine

 [[Image:dC

chemical structure.png|87px|Chemical structure of deoxycytidine]]
Deoxycytidine

 style="text-align: center; border-right: 2px solid grey;"
Inosine
Guanosine
Drug style="text-align: center; border-right: 2px solid black;"
Tenofovir

({[(2R)-1-(6-amino-9H-purin-9-yl)propan-2-yl]oxy}methyl)phosphonic

Chemical structure of zidovudine
Zidovudine 3´Azido-2´,3´-dideoxythymidine, azidothy midine

(AZT)

 style="text-align: center; border-right: 2px solid

grey;"|Chemical structure of Stavudin
Stavudine 2´,3´-Didehydro-2´,3´-dideoxythymidine (d4T)

 Chemical structure of Emticitabine
Emtricitabine

(-)-ß-L-3´-thia-2´,3´-dideoxy-5-fluorocytidine ( (-)FTC)

 Chemical structure of Lamivudine
Lamivudine 2´,3´-Dideoxy-3´-thiacytidine (3TC)
 style="text-align: center; border-right: 2px solid

black;"|Chemical structure of Zalcitabine
Zalcitabine 2´,3´-Dideoxycycytine (ddC)

 style="text-align: center; border-right: 2px solid

grey;"|
Didanosine 2´,3´-Dideoxyinosine (ddI)
Abacavir

(4-(2-amino-6-(cyclopropylamino)-9H-purin-9yl) cyclopent-2enyl)methanol(ABC)

Two main mechanisms are known that cause NRTI drug resistance: Interference with the incorporation of NRTIs and excision of incorporated NRTIsCite error: The <ref> tag has too many names (see the help page).Cite error: The <ref> tag has too many names (see the help page)..Interference with the incorporated NRTIs involves a mutation in the p66 subdomain of the RTCite error: The <ref> tag has too many names (see the help page)..The mutation causes a steric hindrance that can exclude certain drugs, for example lamivudine, from being incorporated during reverse transcription. In case of excision of incorporated NRTIs the resistant enzymes readily accept the inhibitor as a substrate for incorporation into the DNA chainCite error: The <ref> tag has too many names (see the help page).. Subsequently the RT enzyme can remove the incorporated NRTI by reversing the polymerization step. The excision reaction requires a pyrophosphate donor which RT joins to the NRTI at the 3´primer terminus, excising it from the primer DNACite error: The <ref> tag has too many names (see the help page).. To achieve efficient inhibition of HIV-1 replication in patients, and to delay or prevent appearance of drug resistant viruses, drug combinations are used. HAART, also known as highly active antiretroviral therapy consists of combinations of antiviral drugs which include NRTIs, NtRTI, non-nucleoside reverse transcriptase inhibitors and protease inhibitorsCite error: The <ref> tag has too many names (see the help page)..

Current status[edit]

Currently, there are several NRTIs in various stages of clinical and preclinical development. The main reasons for continuing the search for new NRTIs against HIV-1 are to decrease toxicity, increase efficiency against resistant viruses, and simplify anti-HIV-1 treatment[4]Cite error: The <ref> tag has too many names (see the help page).[18].

Apricitabine (ATC)[edit]

Apricitabine is a deoxycytidine analogue. It is structurally related to lamivudine where the positions of the oxygen and the sulfur are essentially reversed[14]. Even though apricitabine is a little less potent in vitro compared to some other NRTIs, it maintains its activity against a broad spectrum of HIV-1 variants with NRTI resistance mutations. Apricitabine is in the final stage of clinical development for the treatment of NRTI-experienced patients[4].

Elvucitabine (L-d4FC)[edit]

Elvucitabine is a deoxycytidine analogue with activity against HIV resistant to several other nucleoside analogues, including zidovudine and lamivudine[15]. This is partly because of high intracellular levels of its triphosphate metabolite reached in cells[4]. Clinical trials of elvucitabine are on hold, because it has shown bone marrow suppression in some patients, with CD4+ cell numbers dropping as early as two days after initiation of dosing[15][18].

Amdoxovir (DAPD)[edit]

Amdoxovir is a guanosine analogue NRTI prodrug that has good bioavailability[4][15][18].It is deaminated intracellularly by adenosine deaminase to dioxolane guanine (DXG). DXG-triphosphate, the active form of the drug, has greater activity than DAPD-triphosphate[15]. Amdoxovir is currently in phasa II clinical trials[17][18].

Racivir (RCV)[edit]

Racivir is a racemic mixture of the two β-enantiomers of emtricitabine (FTC), (-)-FTC and (+)-FTC. Racivir has excellent oral bioavailability and has the advantage of needing to be taken only once a day. Racivir can be considered to be used in combination of two NRTIs and has shown promising antiviral activity when used in combination. Racivir is currently in phase II clinical trials[4][15][18].


Table 4 Several drug candidates that are undergoing clinical development
Drug candidate Apricitabine Elvucitabine Amdoxovir Racivir
Chemical structure
Phase of development Final stage of clinical development On hold phase II phase II


There are several more NRTIs in development. Either the sponsors have filed for an Investigational New Drug (IND) application, the application has been approved by the FDA or the drugs are different phases of clinical trails. Some of the NRTIs that are in development exhibit various attractive pharmacological properties that could make them desirable for the treatment of patients in need of new agents[4][15][18].

See also[edit]

Antiretroviral drug

Discovery and development of CCR5 receptor antagonists

Discovery and Development of Non-Nucleoside Reverse Transcriptase Inhibitors

[[Category:Nucleoside analog reverse transcriptase inhibitors]] [[Category:World Health Organization essential medicines]] [[Category:IARC Group 2B carcinogens]] [[Category:Azides]] [[Category:Tetrahydrofurans]] [[Category:Pyrimidinediones]]

References[edit]

  1. ^ Merson, M.D.; Michael, H. (2006), "HIV–AIDS Pandemic at 25 — The Global Response", The New England Journal of Medicine, 354 (23): 2414–2417, doi:10.1056/NEJMp068074, PMID 16760441
  2. ^ Fausi, A.S. (1999), "The AIDS epidemic Considerations for the 21st Century", 'The New England Journal of Medicine, 351 (14): 1046–1050, doi:10.1056/NEJM199909303411406, PMID 10502595
  3. ^ a b c Fauci, A.S. (2003), "HIV and AIDS: 20 years of science", Nature Medicine, 9 (7): 839–843, doi:10.1038/nm0703-839, PMID 12835701, S2CID 5472960, ProQuest 223114463
  4. ^ a b c d e f g h i j k l Cihlar, T.; Ray, A.S. (2010), "Nucleoside and nucleotide HIV reverse transcriptase inhibitors: 25 years after zidovudine" (PDF), Antiviral Research, 85 (1): 39–58, doi:10.1016/S0149-2918(00)90004-3, PMID 10929917 {{citation}}: Unknown parameter |rm due to error doi= ignored (help)
  5. ^ a b Herschorn, A.; Hizi, A. (2008), "Retroviral reverse transcriptases (other than those of HIV-1 and murine leukemia virus): A comparison of their molecular and biochemical properties" (PDF), Virus Research, 134 (1–2): 203–220, doi:10.1016/j.virusres.2007.12.008, PMID 18291546 {{citation}}: Unknown parameter |rm due to error doi= ignored (help)
  6. ^ a b c Anderson, K.S. (2007), "Reverse transcription of the HIV-1 pandemic", The FASEB Journal, 21 (14): 3795–3808, doi:10.1096/fj.07-8697rev, PMID 17639073, S2CID 24960391{{citation}}: CS1 maint: unflagged free DOI (link)
  7. ^ a b Sneader, W. (1996), Drug prototypes and their exploitation, John Wileys & sons, pp. 448–450, ISBN 978-0471948476
  8. ^ a b c Smith, J.; William, Hywel (1998), Smith and Williams´ Introduction to the principles of drug design and action (3 ed.), Harwood academic publishers, pp. 486–489, ISBN 978-90-5702-037-7
  9. ^ a b Saunders, J. (2000), Top drugs: Top synthetic routes, pp. 71–75
  10. ^ a b c d e Sneader, W. (2005), Drug discovery a history, pp. 250–268, ISBN 100471899798 {{citation}}: Check |isbn= value: length (help)
  11. ^ Georgiev, V.S. (2009), National institute of allergy and infectious diseases, NIH, vol. 2, pp. 417–426, doi:10.1007/978-1-60327-297 (inactive 2022-06-26), ISBN 9781603272694 {{citation}}: Check |isbn= value: checksum (help)CS1 maint: DOI inactive as of June 2022 (link)
  12. ^ a b c De-Clercq, E. (2009), "Anti-HIV drugs: 25 compounds approved within 25 years after the discovery of HIV" (PDF), International Journal of Antimicrobial Agents, 33 (4): 307–320, doi:10.1016/j.ijantimicag.2008.10.010, PMID 19108994
  13. ^ a b Ogden, R.C.; Skowron, G. (2006), Reverse transcriptase inhibitors in HIV/AIDS therapy, Humana press inc, pp. 33–63, ISBN 978-1-58829-649-8
  14. ^ a b c d LaFemina, R.L. (2009), Antiviral research strategies in antiviral drug discovery, AMS press, pp. 51–70
  15. ^ a b c d e f g Otto, M.J. (2003), "New nucleoside reverse transcriptase inhibitors for the treatment of HIV infections" (PDF), Current Opinion in Pharmacology, 9 (7): 839–843, doi:10.1016/j.coph.2004.06.001, PMID 15351346
  16. ^ a b Fung, H.B.; Piacenti, F.J.; Stone, E.A. (2002), "Tenofovir Disoproxil Fumarate: A Nucleotide ReverseTranscriptase Inhibitor for the Treatment of HIV Infection" (PDF), Clinical Therapeutics, 24 (10): 1515–1548, doi:10.1016/S0149-2918(02)80058-3, PMID 12462284 {{citation}}: Unknown parameter |rm due to error doi= ignored (help)
  17. ^ a b c Nguyen-Ba, N.; Rando, R.F. (2000), "Development of novel nucleoside analogues for use against drug resistant strains of HIV-1" (PDF), Drug Discovery Today, 5 (10): 465–476, doi:10.1016/S1359-6446(00)01558-0, PMID 11018598
  18. ^ a b c d e f Agrawala, R.K.; Krishnan, P.N.; Raman, S.; Ravichandran, S.; Veerasamy, R. (2008), "An overview on HIV-1 reverse transcriptase inhibitors" (PDF), Digest Journal of Nanomaterials and Biostructures, 3 (4): 171–187



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