The 2024–25 LEN Challenger Cup was the 3rd edition of a water polo competition organised by European Aquatics. It acts as the third tier, below the LEN Champions League and the LEN Euro Cup. The reigning champions are Galatasaray, but they won't defend their title as they didn't enter.
The draw was on 16 September 2024.[4] H indicates which club is hosting the groups. Teams in bold advanced to the quarter-finals. The seeding was decided by as follows:
The seeded and unseeded clubs consist of the teams that finished first and second in the previous round respectively. The only restriction was that clubs from the same group in the previous round could not be drawn against each other.