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Aliphatic (R)-hydroxynitrile lyase

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Aliphatic (R)-hydroxynitrile lyase
Identifiers
EC no.4.1.2.46
Databases
IntEnzIntEnz view
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Aliphatic (R)-hydroxynitrile lyase (EC 4.1.2.46, (R)-HNL, (R)-oxynitrilase, (R)-hydroxynitrile lyase, LuHNL) is an enzyme with systematic name (2R)-2-hydroxy-2-methylbutanenitrile butan-2-one-lyase (cyanide forming).[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction:

(2R)-2-hydroxy-2-methylbutanenitrile cyanide + butan-2-one

The enzyme contains Zn2+.

References

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  1. ^ Trummler, K.; Roos, J.; Schwaneberg, U.; Effenberger, F.; Förster, S.; Pfizenmaier, K.; Wajant, H. (1998). "Expression of the Zn2+-containing hydroxynitrile lyase from flax (Linum usitatissimum) in Pichia pastoris— utilization of the recombinant enzyme for enzymatic analysis and site-directed mutagenesis". Plant Sci. 139: 19–27. doi:10.1016/s0168-9452(98)00173-3.
  2. ^ Trummler, K.; Wajant, H. (1997). "Molecular cloning of acetone cyanohydrin lyase from flax (Linum usitatissimum). Definition of a novel class of hydroxynitrile lyases". J. Biol. Chem. 272 (8): 4770–4774. doi:10.1074/jbc.272.8.4770. PMID 9030531.
  3. ^ Albrecht J, Jansen I, Kula MR (1993). "Improved purification of an (R)-oxynitrilase from Linum usitatissimum (flax) and investigation of the substrate range". Biotechnol. Appl. Biochem. 17 (2): 191–203. PMID 8387315.
  4. ^ Xu, L.-L.; Singh, B.K.; Conn, E.E. (1988). "Purification and characterization of acetone cyanohydrin lyase from Linum usitatissimum". Arch. Biochem. Biophys. 263 (2): 256–263. doi:10.1016/0003-9861(88)90634-0. PMID 3377504.
  5. ^ Cabirol, F.L.; Tan, P.L.; Tay, B.; Cheng, S.; Hanefeld, U.; Sheldon, R.A. (2008). "Linum usitatissimum hydroxynitrile lyase cross-linked enzyme aggregates: a recyclable enantioselective catalyst". Adv. Synth. Catal. 350 (14–15): 2329–2338. doi:10.1002/adsc.200800309.
  6. ^ Breithaupt, H.; Pohl, M.; Bönigk, W.; Heim, P.; Schimz, K.-L.; Kula, M.-R. (1999). "Cloning and expression of (R)-hydroxynitrile lyase from Linum usitatissimum (flax)". J. Mol. Catal. B. 6 (3): 315–332. doi:10.1016/S1381-1177(98)00109-X.
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