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Draft:Biotium

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  • Comment: Certain significant part of the draft weren't sourced properly. The "history" section is a crucial part of the significance, and needs adequate reliable sources. Safari ScribeEdits! Talk! 23:17, 23 July 2024 (UTC)
  • Comment: Also remove all external links in the body and be sure any sources you cite do not have a conflict of interest with Biotium Inc. and have written about Biotium Inc. (the company) in-depth. S0091 (talk) 21:21, 23 July 2024 (UTC)


Biotium

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Biotium Inc.
Industry: Biotechnology
Founded: 2001
Headquarters: Fremont, California, USA
Area served: Worldwide
Key People: Fei Mao, Ph.D., Founder, President and CTO; Vivien Chen, M.B.A., co-founder and CCO
Products: Fluorescent dyes, Cell biology reagents, Molecular biology reagents, Cellular stains, Viability stains, Antibodies, Extracellular Vesicle research products, Custom Product Development, Viability PCR
Website: https://biotium.com/

Biotium Inc. is a privately held American life science reagent manufacturer and supplier founded in 2001[1]. Biotium specializes in providing fluorescent reagents for use in microscopy, flow cytometry, or molecular biology applications.[2][3][4] The company is located in the Bay Area. Biotium’s collaborative R&D teams include chemists and biologists who act synergistically to design and validate novel fluorescent dyes for use in life science and medical research.

Business segments

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Biotium’s life science products predominantly include fluorescent reagents and consumables for cell or molecular biology, or protein analysis. In the early 2000’s the company was early in offering nucleic acid stains that are safer for the user than other common options[5][6][7], GelRed® and GelGreen®, and these products remain commonly-used in both research and educational settings. EvaGreen® Dye is another commonly-used molecular biology product developed by Biotium that offers an alternative to SYBR Green and has proven useful in PCR and HRM research as well as in molecular diagnostics and forensics protocols.[8][9][10]

The company also developed its line of around 40 CF® Dye fluorophores for use in varied applications; CF® Dyes have since been incorporated into a variety of kits, reactive options, and labeled antibodies.[3] Various CF® Dyes have also now been shown by third-party researchers to be compatible with super-resolution microscopy methodologies, including STORM. [11][12] Biotium has also developed and offers live cell fluorescent stains for subcellular structures or cellular processes, such as NucView® for real-time apoptosis detection.[13][14]

Viability PCR (v-PCR) reagents are another specialty of Biotium, and they helped develop and are currently a major global supplier of propidium monoazide (PMA) for use in v-PCR[15]. Biotium scientists also invented an alternative to PMA, named PMAxx™, that has been shown to be more effective than PMA at eliminating false positives in certain bacterial viability assays.[16][17][18][19] Peer-reviewed publications have also shown that PMA and PMAxx™ are useful in the monitoring viable bacteria in the environment and for assessing viral capsid integrity in DNA and RNA viruses.[20][21] Biotium also sells select equipment and accessory consumables. Their sales platforms include direct-to-customer sales for domestic customers and domestic and international distributors.

History

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Biotium was founded in 2001, by Fei Mao Ph.D., a graduate of the University of Oregon, and co-founder Vivien Chen MBA.[22][3][23][24] Since then, Biotium researchers have developed over 30 patented technologies[25]. Many of these patented technologies have been licensed to other life science technology companies. The company is currently headquartered in Fremont, California[1].

References

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  1. ^ a b Dun & Bradstreet. "Dun & Bradstreet". D&B Business Directory. Dun & Bradstreet. Retrieved 26 July 2024.
  2. ^ Connon, Catherine Cupples (2023). Forensic DNA Analysis: Methods and Protocols (Vol. 2685 ed.). Springer Nature. p. 440. ISBN 978-1071632949.
  3. ^ a b c Kist, Tarso; Ledur, B. (2023). Fluorescent Dye Labels and Stains: A Database of Photophysical Properties. John Wiley & Sons. p. 480. ISBN 978-1-119-83513-4.
  4. ^ Goetz, Christine; Hammerbeck, Christopher; Bonnevier, Jody (2018). Flow cytometry basics for the non-expert (Vol. 1 ed.). Cham, Switzerland: Springer. p. 240. ISBN 978-3319980706.
  5. ^ Haines, Alicia; Tobe, Shanan; Kobus, Hilton; Linacre, Adrian (2015). "Properties of nucleic acid staining dyes used in gel electrophoresis". Electrophoresis. 36 (6): 941–944. doi:10.1002/elps.201400496. PMID 25546455.
  6. ^ Huang, Qing; Baum, Larry Baum; Fu, Wei-Ling (2010). "Simple and practical staining of DNA with GelRed in agarose gel electrophoresis". Clinical Laboratories. 56 (3): 149–152. PMID 20476647.
  7. ^ Anjomshoa, Marzieh; Torkzadeh-Mahani, Masoud (2016). "Competitive DNA-binding studies between metal complexes and GelRed as a new and safe fluorescent DNA dye". Journal of Fluorescence. 26 (4): 1505–1510. doi:10.1007/s10895-016-1850-z. PMID 27324950.
  8. ^ Cheng, Juhui; Jiang, Yonghou; Rao, Pinbin; Wu, Haigang; Dong, Qinfang; Wu, Zhiyi; Ding, Xianfeng; Guo, Jiangfeng (2013). "Development of a single-tube multiplex real-time PCR for detection and identification of five pathogenic targets by using melting-curve analysis with EvaGreen". Archives of Virology 158 (2013). 158 (2): 379–386. doi:10.1007/s00705-012-1493-6. PMID 23053522.
  9. ^ Eischeid, Anne (2011). "SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR". BMC Research Notes. 4: 263. doi:10.1186/1756-0500-4-263. PMC 3162529. PMID 21798028.
  10. ^ Mao, Fei; Leung, Wai-Yee; Xin, Xing (2007). "Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications". BMC Biotechnology. 7: 76. doi:10.1186/1472-6750-7-76. PMC 2213645. PMID 17996102.
  11. ^ Bowler, Mathew; Kong, Dong; Sun, Shufeng; Nanjundappa, Rashmi; Evans, Lauren; Farmer, Veronica; Holland, Andrew; Mahjoub, Moe R.; Sui, Haixin; Jadranka, Loncarek (2019). "High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy". Nature Communications. 10 (1): 993. Bibcode:2019NatCo..10..993B. doi:10.1038/s41467-018-08216-4. PMC 6397210. PMID 30824690.
  12. ^ Wang, Bowen; Xiong, Michael; Susanto, Josephine; Li, Xue; Leung, Wai-Yee; Xu, Ke (2022). "Transforming Rhodamine Dyes for (d) STORM Super-Resolution Microscopy via 1, 3-Disubstituted Imidazolium Substitution". Angewandte Chemie International Edition. 61 (9): e202113612. doi:10.1002/anie.202113612. PMID 34919772.
  13. ^ Cen, Hui; Mao, Fei; Aronchik, Ida; Fuentes, Rholinelle Joy; Firestone, Gary L. (2008). "DEVD-NucView488: A novel class of enzyme substrates for real-time detection of caspase-3 activity in live cells". The FASEB Journal 22, No. 7 (2008). 22 (7): 2243–2252. doi:10.1096/fj.07-099234. PMID 18263700.
  14. ^ Alvero, Ayesha B.; Mor, Gil G. (2021). Alvero, Ayesha B., Gil G. Mor, and Alvero. Detection of Cell Death Mechanisms. Springer US, 2021. Springer US. p. 256. doi:10.1007/978-1-0716-1162-3. ISBN 978-1071611647.
  15. ^ Nocker, Andreas; Cheung, Ching-Ying; Camper, Anne (2006). "Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells". Journal of Microbiological Methods. 67 (2): 310–320. doi:10.1016/j.mimet.2006.04.015. PMID 16753236.
  16. ^ Lv, Xinrui; Gu, Xiaokui; Wang, Li; He, Xiaoxin; He, Chunai; Zhang, Jingfeng; Zhao, Lichao (2021). "Rapid and absolute quantification of VBNC Cronobacter sakazakii by PMAxx combined with single intact cell droplet digital PCR in infant foods". LWT. 145: 111388. doi:10.1016/j.lwt.2021.111388.
  17. ^ Huang, Tiantian; Shi, Yaoqiang; Zhang, Jinyang; J, Huang; Han, Qinqin; Xia, Xue-shan; Zhang, A-Mei; Song, Yuzhu Song (2021). "Rapid and simultaneous detection of five, viable, foodborne pathogenic bacteria by photoinduced PMAxx-coupled multiplex PCR in fresh juice". Foodborne Pathogens and Disease. 18 (9): 640–646. doi:10.1089/fpd.2020.2909. PMID 34292761.
  18. ^ Randazzo, Walter; Piqueras, Joaquín; Rodríguez-Díaz, Jesús; Aznar, Rosa; Sánchez, G. (2018). "Improving efficiency of viability-qPCR for selective detection of infectious HAV in food and water samples". Journal of Applied Microbiology. 124 (4): 958–964. doi:10.1111/jam.13519. PMID 28649706.
  19. ^ Wen, Yuanyi; Tan, Youjiang; Zhao, Lichao; Lv, Xinrui; Lin, Li; Liang, Dezhi; Wang, Li (2022). "Rapid on-site detection of viable Escherichia coli O157: H7 in lettuce using immunomagnetic separation combined with PMAxx-LAMP and nucleic acid lateral flow strip". Microchemical Journal. 178: 107348. doi:10.1016/j.microc.2022.107348.
  20. ^ Oristo, S.; Lee, H.J.; Maunula, L. (2018). "Performance of pre-RT-qPCR treatments to discriminate infectious human rotaviruses and noroviruses from heat-inactivated viruses: applications of PMA/PMAxx, benzonase and RNase". Journal of Applied Microbiology. 124 (4): 1008–1016. doi:10.1111/jam.13737. PMID 29464825.
  21. ^ Pedrosa de Macena, Lorena da Graça; Simone de Oliveira Pereira, Joseane; César Ferreira, Fernando; Gonçalves Maranhão, Adriana; Maria Lanzarini, Natália; Miagostovich, Marize Pereira (2022). "Quantification of infectious Human mastadenovirus in environmental matrices using PMAxx-qPCR". Brazilian Journal of Microbiology. 53 (3): 1465–1471. doi:10.1007/s42770-022-00775-5. PMC 9168632. PMID 35666431.
  22. ^ Heller, Adam; Feldman, Ben (2010). "Electrochemistry in diabetes management". Accounts of Chemical Research. 43 (7): 963–973. doi:10.1021/ar9002015. PMID 20384299.
  23. ^ Gaffield, Michael; Betz, William (2006). "Imaging synaptic vesicle exocytosis and endocytosis with FM dyes". Nature Protocols. 1 (6): 2916–2921. doi:10.1038/nprot.2006.476. PMID 17406552.
  24. ^ Staff reporter (2017). "Biotech entrepreneur to speak at distinguished alumni event".
  25. ^ "Patent Public Search Basic | USPTO". Patent Public Search Basic (PPUBS Basic). Retrieved 24 July 2024.
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