Hari Sharan Karki
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Hari Sharan Karki is an American plant pathologist and microbiologist specializing in plant-microbe interactions, particularly focusing on rice and soybean pathogens. He has held research positions at Louisiana State University (LSU) and the United States Department of Agriculture (USDA).[1][2]
Education
[edit]Karki earned a Master of Science (MS) degree in Plant Pathology and Crop Physiology from Louisiana State University in 2010. His master's thesis was titled "Physiological, biochemical and molecular characteristics associated with virulence of Burkholderia glumae: the major causative agent of bacterial panicle blight of rice". He continued his studies at LSU, completing a Doctor of Philosophy (PhD) in the same field in 2013 with a dissertation titled "A Genetic Study on the Virulence Mechanism of Burkholderia glumae and Rice Resistance to Bacterial Panicle Blight and Sheath Blight".[2][3]
Career
[edit]Karki has been involved in research roles at LSU and the USDA, focusing on understanding plant diseases and developing disease-resistant crop varieties. His work primarily addresses the challenges posed by bacterial pathogens affecting economically important crops such as rice and soybean. [3]
Research
[edit]Karki's research centers on the bacterial pathogen Burkholderia glumae, which causes bacterial panicle blight (BPB) in rice. His studies have explored the physiological, biochemical, and molecular characteristics associated with the virulence of B. glumae. He has identified key virulence factors, including toxoflavin, lipase, and motility, and investigated the role of quorum-sensing signals in pathogen virulence.[4]
In his doctoral research, Karki conducted a genetic study on the virulence mechanisms of B. glumae and rice resistance to BPB and sheath blight. He identified several regulatory elements involved in the pathogen's virulence, including sensor histidine kinase and response regulators. Additionally, he developed high-throughput methods for virulence testing using onion bulb scales as an alternative to rice panicles, facilitating more efficient preliminary assessments of pathogen virulence.[5][6]
Beyond rice pathology, Karki has contributed to soybean and potato research. His work in soybean pathology involves identifying natural genetic diversity that confers resistance to various pathogens. In potato research, he has focused on resistance to Phytophthora infestans, the causative agent of late blight, identifying new sources of resistance in wild potato species.[7][8][9]
Publications
[edit]Karki has authored and co-authored several scholarly works related to plant pathology and microbiology. His publications include detailed studies on the virulence mechanisms of Burkholderia glumae, the development of disease-resistant crop varieties, and the genetic basis of plant resistance to bacterial diseases.[3]
References
[edit]- ^ "Hari Karki : USDA ARS". www.ars.usda.gov. Retrieved 2024-09-04.
- ^ a b "Hari Sharan Karki, Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, USA — BIO-PROTOCOL". bio-protocol.org. Retrieved 2024-09-04.
- ^ a b c Shrestha, Bishnu K.; Karki, Hari Sharan; Groth, Donald E.; Jungkhun, Nootjarin; Ham, Jong Hyun (2016-01-14). "Biological Control Activities of Rice-Associated Bacillus sp. Strains against Sheath Blight and Bacterial Panicle Blight of Rice". PLOS ONE. 11 (1): e0146764. Bibcode:2016PLoSO..1146764S. doi:10.1371/journal.pone.0146764. ISSN 1932-6203. PMC 4713167. PMID 26765124.
- ^ Chen, Ruoxi; Barphagha, Inderjit K.; Karki, Hari S.; Ham, Jong Hyun (2012-12-20). "Dissection of Quorum-Sensing Genes in Burkholderia glumae Reveals Non-Canonical Regulation and the New Regulatory Gene tofM for Toxoflavin Production". PLOS ONE. 7 (12): e52150. Bibcode:2012PLoSO...752150C. doi:10.1371/journal.pone.0052150. ISSN 1932-6203. PMC 3527420. PMID 23284909.
- ^ Karki, Hari (2010-01-01). "Physiological, biochemical and molecular characteristics associated with virulence of Burkholderia glumae: the major causative agent of bacterial panicle blight of rice". LSU Master's Theses. doi:10.31390/gradschool_theses.786.
- ^ Ontoy, John Christian; Shrestha, Bishnu; Karki, Hari Sharan; Barphagha, Inderjit; Angira, Brijesh; Famoso, Adam; Ham, Jong Hyun (January 2023). "Genetic Characterization of the Partial Disease Resistance of Rice to Bacterial Panicle Blight and Sheath Blight by Combined QTL Linkage and QTL-seq Analyses". Plants. 12 (3): 559. doi:10.3390/plants12030559. ISSN 2223-7747. PMC 9920235. PMID 36771643.
- ^ Karki, Hari S.; Abdullah, Sidrat; Chen, Yu; Halterman, Dennis A. (September 2021). "Natural Genetic Diversity in the Potato Resistance Gene RB Confers Suppression Avoidance from Phytophthora Effector IPI-O4". Molecular Plant-Microbe Interactions. 34 (9): 1048–1056. doi:10.1094/MPMI-11-20-0313-R. ISSN 0894-0282. PMID 33970667.
- ^ bio-protocol.org https://bio-protocol.org/en/bpdetail?id=3926&type=0. Retrieved 2024-09-04.
{{cite web}}: Missing or empty|title=(help) - ^ Karki, Hari S.; Jansky, Shelly H.; Halterman, Dennis A. (February 2021). "Screening of Wild Potatoes Identifies New Sources of Late Blight Resistance". Plant Disease. 105 (2): 368–376. doi:10.1094/PDIS-06-20-1367-RE. ISSN 0191-2917. PMID 32755364.