CIP2A
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| Aliases | CIP2A, C330027C09Rik, AA408511, AU018569, Cip2a, Kiaa1524, p90, KIAA1524, cell proliferation regulating inhibitor of protein phosphatase 2A, cellular inhibitor of PP2A, NOCIVA | ||||||||||||||||||||||||||||||||||||||||||||||||||
| External IDs | OMIM: 610643; MGI: 2146335; HomoloGene: 10842; GeneCards: CIP2A; OMA:CIP2A - orthologs | ||||||||||||||||||||||||||||||||||||||||||||||||||
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Protein CIP2A also known as cancerous inhibitor of PP2A (CIP2A) is a protein that in humans is encoded by the KIAA1524 gene.[5][6]
CIP2A is a regulatory protein involved in the inhibition of the serine-threonine phosphatase activity of Protein phosphatase 2A (PP2A). PP2A is a trimeric enzyme composed of a catalytic C-subunit, a scaffolding A-subunit, and various regulatory B-subunits, which collectively dephosphorylate a vast majority of cellular serine/threonine phosphorylated proteins, including many involved in cancer progression. CIP2A has been shown to regulate phosphorylation and activity of numerous oncoproteins, promoting malignant cell growth and tumorigenesis in various human cancers. High expression levels of CIP2A have been observed in multiple cancer types, such as head and neck cancer, colon cancer, gastric cancer, breast cancer, prostate cancer, and lung cancer, correlating with poor patient prognosis and disease aggressivity. Additionally, CIP2A is implicated in resistance to cancer treatments, such as imatinib in chronic myeloid leukemia.
Function
[edit]Protein phosphatase 2A (PP2A) is a trimeric serine-threonine phosphatase consisting of a catalytic C-subunit (PP2Ac), a scaffolding A-subunit and various regulatory B-subunits. Importantly, it has been estimated that collectively PP2A complexes can dephosphorylate a vast majority of all cellular serine/threonine phosphorylated proteins including large number of phosphoproteins involved in cancer maintenance and progression. The functional role of PP2A as a human tumor suppressor was validated by studies initiated by the Weinberg laboratory, which demonstrated that normal human cells immortalized by overexpression of TERT and inhibition of p53 and Rb, could not be transformed by oncogenic forms of H-Ras without simultaneous inhibition of PP2A activity. Increased activity of oncogenic kinases is not sufficient to drive human cell transformation if PP2A activity is not simultaneously inhibited. In striking contrast to the tumor suppressor p53, which in human tumors is mainly inactivated by mutations, PP2A complex proteins are mutated at low frequency and rather seem to be inhibited by overexpression of PP2A inhibitor proteins such as CIP2A, PME-1 and SET.[7]
Role in meiosis
[edit]A unique feature of meiosis in female mammals, not seen in other cell types, is the characteristic prolonged arrest during the prophase stage of meiosis I.[8] Specifically in oocytes, DNA double-strand breaks can be repaired during meiosis I by a mechanism involving microtubule-dependent recruitment from spindle pole to chromosomes of a protein complex composed of CIP2A (the protein encoded by KIAA1524 gene), MDC1 and TOPBP1.[8]
Clinical significance
[edit]CIP2A has been shown to regulate phosphorylation and activity of many other oncoproteins and to drive malignant cell growth and tumorigenesis in various human cancer types. In a recent phosphoproteome study CIP2A depletion was shown to induce dephosphorylation of more than a hundred phosphorylation sites in proteins that were involved in a wide range of cellular functions.[9] This and other published data indicate that CIP2A's role in cancer is linked to many other targets beyond MYC and that CIP2A has important roles in many cellular processes. One example of a recently identified CIP2A regulated phosphoprotein is PKM2. CIP2A was shown in that study to promote PKM2 tetramer formation and oxidative phosphorylation in non-small cell lung cancer.[10] Importantly, CIP2A deficient mice are viable and do not display any life-threatening spontaneous phenotypes,[11] suggesting that targeting of oncogenic function of CIP2A would not result in serious side-effects.
CIP2A is over-expressed in several common human malignancies including human head and neck squamous cell carcinoma (HNSCC), colon cancer, gastric cancer, breast cancer, prostate cancer, and lung cancer. Notably, in these cancer types CIP2A over-expression is observed with very high frequency; in breast cancer around 40% of cancer patients are over-expressing CIP2A whereas in all other studied cancer types the frequency is between 65 and 87%. In breast cancer, CIP2A expression correlates with disease aggressivity whereas in gastric and lung cancer CIP2A expression predicts poor patient survival.[12][13] To date, high CIP2A expression has been observed to predict poor patient prognosis in more than a dozen human cancer types, which makes it one of the most frequently altered human oncoproteins with clinical relevance.
CIP2A is also over-expressed in prostate cancer,[14] lung cancer,[15] oral squamous cell carcinoma,[16] and gastric cancer.[12] Furthermore, the expression of CIP2A correlates with breast cancer aggressivity.[17] It is also implicated in some Chronic Myeloid Leukemia (CML) resistance to imatinib (Gleevec).
History
[edit]In a search for potential non-genetic mechanisms that could inhibit PP2A in human cancer cells, the Westermarck laboratory at the University of Turku (Turku, Finland) used affinity purification coupled with mass spectrometry proteomics (AP-MS) to identify interaction partners for the A-subunit of PP2A.[18] In this PP2A protein complex, they identified a known PP2A inhibitor protein PME1 and a protein named p90 (gene KIAA1524), which the laboratory of Edward Chan at The Scripps Research Institute (La Jolla, California, US) had recently reported to be overexpressed in a small number of gastric cancer patient samples.[19] No function for this protein had been reported.
In their study, Junttila, Puustinen, and co-workers demonstrated that CIP2A inhibits PP2A activity towards the oncogenic transcription factor c-Myc, thereby preventing c-Myc proteolytic degradation. CIP2A was required for malignant cellular growth and in vivo tumor formation. Overexpression of CIP2A promotes Ras-elicited cell growth and transforms immortalized human cells (HEK-TERVs). CIP2A was also shown to be overexpressed in human and mouse head and neck squamous cell carcinoma (HNSCC) as well as human colon cancer. Based on these characteristics, the protein was renamed as cancerous inhibitor of PP2A (CIP2A).
References
[edit]- ^ a b c GRCh38: Ensembl release 89: ENSG00000163507 – Ensembl, May 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000033031 – Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Nagase T, Kikuno R, Ishikawa K, Hirosawa M, Ohara O (April 2000). "Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Research. 7 (2): 143–150. doi:10.1093/dnares/7.2.143. PMID 10819331.
- ^ "Entrez Gene: KIAA1524 KIAA1524".
- ^ Seshacharyulu P, Pandey P, Datta K, Batra SK (July 2013). "Phosphatase: PP2A structural importance, regulation and its aberrant expression in cancer". Cancer Letters. 335 (1): 9–18. doi:10.1016/j.canlet.2013.02.036. PMC 3665613. PMID 23454242.
- ^ a b Leem J, Kim JS, Oh JS (June 2023). "Oocytes can repair DNA damage during meiosis via a microtubule-dependent recruitment of CIP2A-MDC1-TOPBP1 complex from spindle pole to chromosomes". Nucleic Acids Research. 51 (10): 4899–4913. doi:10.1093/nar/gkad213. PMC 10250218. PMID 36999590.
- ^ Kauko O, Imanishi SY, Kulesskiy E, Yetukuri L, Laajala TD, Sharma M, et al. (March 2020). "Phosphoproteome and drug-response effects mediated by the three protein phosphatase 2A inhibitor proteins CIP2A, SET, and PME-1". The Journal of Biological Chemistry. 295 (13): 4194–4211. doi:10.1074/jbc.RA119.011265. PMC 7105317. PMID 32071079.
- ^ Liang LJ, Yang FY, Wang D, Zhang YF, Yu H, Wang Z, et al. (February 2024). "CIP2A induces PKM2 tetramer formation and oxidative phosphorylation in non-small cell lung cancer". Cell Discovery. 10 (1): 13. doi:10.1038/s41421-023-00633-0. PMC 10847417. PMID 38321019.
- ^ Ventelä S, Côme C, Mäkelä JA, Hobbs RM, Mannermaa L, Kallajoki M, et al. (2012). "CIP2A promotes proliferation of spermatogonial progenitor cells and spermatogenesis in mice". PLOS ONE. 7 (3): e33209. Bibcode:2012PLoSO...733209V. doi:10.1371/journal.pone.0033209. PMC 3312892. PMID 22461891.
- ^ a b Khanna A, Böckelman C, Hemmes A, Junttila MR, Wiksten JP, Lundin M, et al. (June 2009). "MYC-dependent regulation and prognostic role of CIP2A in gastric cancer". Journal of the National Cancer Institute. 101 (11): 793–805. doi:10.1093/jnci/djp103. PMID 19470954.
- ^ Soo Hoo L, Zhang JY, Chan EK (July 2002). "Cloning and characterization of a novel 90 kDa 'companion' auto-antigen of p62 overexpressed in cancer". Oncogene. 21 (32): 5006–5015. doi:10.1038/sj.onc.1205625. PMID 12118381.
- ^ Vaarala MH, Väisänen MR, Ristimäki A (October 2010). "CIP2A expression is increased in prostate cancer". Journal of Experimental & Clinical Cancer Research. 29 (1): 136. doi:10.1186/1756-9966-29-136. PMC 2984408. PMID 20964854.
- ^ Dong QZ, Wang Y, Dong XJ, Li ZX, Tang ZP, Cui QZ, et al. (March 2011). "CIP2A is overexpressed in non-small cell lung cancer and correlates with poor prognosis". Annals of Surgical Oncology. 18 (3): 857–865. doi:10.1245/s10434-010-1313-8. PMID 20842459. S2CID 10445927.
- ^ Basile JR, Czerninski R (October 2010). "The role of CIP2A in oral squamous cell carcinoma". Cancer Biology & Therapy. 10 (7): 700–702. doi:10.4161/cbt.10.7.13151. PMID 20729627.
- ^ Côme C, Laine A, Chanrion M, Edgren H, Mattila E, Liu X, et al. (August 2009). "CIP2A is associated with human breast cancer aggressivity". Clinical Cancer Research. 15 (16): 5092–5100. doi:10.1158/1078-0432.CCR-08-3283. PMID 19671842.
- ^ Junttila MR, Puustinen P, Niemelä M, Ahola R, Arnold H, Böttzauw T, et al. (July 2007). "CIP2A inhibits PP2A in human malignancies". Cell. 130 (1): 51–62. doi:10.1016/j.cell.2007.04.044. PMID 17632056. S2CID 7748174.
- ^ Soo Hoo L, Zhang JY, Chan EK (July 2002). "Cloning and characterization of a novel 90 kDa 'companion' auto-antigen of p62 overexpressed in cancer". Oncogene. 21 (32): 5006–5015. doi:10.1038/sj.onc.1205625. PMID 12118381.
Further reading
[edit]- Chen KF, Liu CY, Lin YC, Yu HC, Liu TH, Hou DR, et al. (November 2010). "CIP2A mediates effects of bortezomib on phospho-Akt and apoptosis in hepatocellular carcinoma cells". Oncogene. 29 (47): 6257–6266. doi:10.1038/onc.2010.357. PMID 20729919.
- Guenebeaud C, Goldschneider D, Castets M, Guix C, Chazot G, Delloye-Bourgeois C, et al. (December 2010). "The dependence receptor UNC5H2/B triggers apoptosis via PP2A-mediated dephosphorylation of DAP kinase". Molecular Cell. 40 (6): 863–876. doi:10.1016/j.molcel.2010.11.021. PMID 21172653.
- Laine A, Sihto H, Come C, Rosenfeldt MT, Zwolinska A, Niemelä M, et al. (February 2013). "Senescence sensitivity of breast cancer cells is defined by positive feedback loop between CIP2A and E2F1". Cancer Discovery. 3 (2): 182–197. doi:10.1158/2159-8290.CD-12-0292. PMC 3572190. PMID 23306062.
- Lucas CM, Harris RJ, Giannoudis A, Copland M, Slupsky JR, Clark RE (June 2011). "Cancerous inhibitor of PP2A (CIP2A) at diagnosis of chronic myeloid leukemia is a critical determinant of disease progression". Blood. 117 (24): 6660–6668. doi:10.1182/blood-2010-08-304477. PMID 21490338.
- Sung WW, Wang YC, Lin PL, Cheng YW, Chen CY, Wu TC, et al. (August 2013). "IL-10 promotes tumor aggressiveness via upregulation of CIP2A transcription in lung adenocarcinoma". Clinical Cancer Research. 19 (15): 4092–4103. doi:10.1158/1078-0432.CCR-12-3439. PMID 23743567.
- Tseng LM, Liu CY, Chang KC, Chu PY, Shiau CW, Chen KF (April 2012). "CIP2A is a target of bortezomib in human triple negative breast cancer cells". Breast Cancer Research. 14 (2): R68. doi:10.1186/bcr3175. PMC 3446403. PMID 22537901.