m7G(5')pppN diphosphatase
| m7G(5')pppN diphosphatase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.6.1.59 | ||||||||
| CAS no. | 82599-75-5 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
In enzymology, a m7G(5')pppN diphosphatase (EC 3.6.1.59) is an enzyme that catalyzes the chemical reaction
- 7-methylguanosine 5'-triphospho-5'-polyribonucleotide + H2O 7-methylguanosine 5'-phosphate + 5'-diphospho-polyribonucleotide
Thus, the two substrates of this enzyme are 7-methylguanosine 5'-triphospho-5'-polynucleotide and H2O, whereas its two products are 7-methylguanosine 5'-phosphate and 5'-diphospho-polyribonucleotide.[1][2][3]
This enzyme removes the N7-methylguanosine 5-phosphate cap from an mRNA. The process of mRNA decapping controls eukaryotic mRNA degradation.[4]
This is the enzyme involved in the processing of amphetamines of the cathinone group, including mephedrone and khat.
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing anhydrides. The systematic name of this enzyme class is 7-methylguanosine-5'-triphospho-5'-polynucleotide 7-methylguanosine-5'-phosphohydrolase. Other names in common use include decapase, and m7G(5')pppN pyrophosphatase.
See also
[edit]References
[edit]- ^ Nuss, Donald L.; Furuichi, Yasuhiro (1977). "Characterization of the m7G(5')pppN-pyrophosphatase activity from HeLa cells". Journal of Biological Chemistry. 252 (9): 2815–2821. doi:10.1016/S0021-9258(17)40435-2. PMID 16003.
- ^ Lavers, Gene C. (1977). "Cleavage of pm7G from mRNA 5'terminal cap structures by pyrophosphatase activity in embryonic chick lens cells". Molecular Biology Reports. 3 (6): 413–420. doi:10.1007/BF00808382. PMID 593271.
- ^ Nuss, Donald L.; Altschuler, Robert E.; Peterson, Andrew J. (1982). "Purification and characterization of the m7G(5')pppN-pyrophosphatase from human placenta". Journal of Biological Chemistry. 257 (11): 6224–6230. doi:10.1016/S0021-9258(20)65128-6. PMID 6122684.
- ^ Furuichi, Yasuhiro (2015). "Discovery of m7G-cap in eukaryotic mRNAs". Proceedings of the Japan Academy, Series B. 91 (8): 394–409. doi:10.2183/pjab.91.394. PMC 4729855. PMID 26460318.
