Methanosarcina sRNAs
sRNA162, sRNA154, sRNA41 are small non-coding RNA (sRNA) identified together with 248 other sRNA candidates by RNA sequencing in methanogenic archaeon Methanosarcina mazei Gö1.[1] These sRNAs were further characterised. It was shown that sRNA162 can interact with both, a cis- and a trans-encoded mRNAs using two distinct domains. The sRNA overlaps the 5′UTR of the MM2442 mRNA and acts as a cis-encoded antisense RNA, and it also regulates MM2441 expression as a trans-encoded sRNA. It exhibits a regulatory role in the metabolic switch between methanol and trimethylamine as carbon and energy source. [2] sRNA154, exclusively expressed under nitrogen deficiency, has a central regulatory role in nitrogen metabolism affecting nitrogenase and glutamine synthetase by masking the ribosome binding site or positively affecting transcript stability. [3] sRNA41, highly expressed during nitrogen sufficiency, is capable to bind several ribosome binding sites independently within a polycistronic mRNA. It was proposed to inhibits translation initiation of all ACDS (acetyl-CoA decarbonylase/synthase complex) genes in N-dependent manner. [4]
See also[edit]
Bacterial sRNA involved in nitrogen metabolism: NsiR4
Other archaeal sRNAs:
References[edit]
- ^ Jäger D, Sharma CM, Thomsen J, Ehlers C, Vogel J, Schmitz RA (December 2009). "Deep sequencing analysis of the Methanosarcina mazei Gö1 transcriptome in response to nitrogen availability". Proceedings of the National Academy of Sciences of the United States of America. 106 (51): 21878–21882. Bibcode:2009PNAS..10621878J. doi:10.1073/pnas.0909051106. PMC 2799843. PMID 19996181.
- ^ Jäger D, Pernitzsch SR, Richter AS, Backofen R, Sharma CM, Schmitz RA (November 2012). "An archaeal sRNA targeting cis- and trans-encoded mRNAs via two distinct domains". Nucleic Acids Research. 40 (21): 10964–10979. doi:10.1093/nar/gks847. PMC 3510493. PMID 22965121.
- ^ Prasse D, Förstner KU, Jäger D, Backofen R, Schmitz RA (November 2017). "154 a newly identified regulator of nitrogen fixation in Methanosarcina mazei strain Gö1". RNA Biology. 14 (11): 1544–1558. doi:10.1080/15476286.2017.1306170. PMC 5785227. PMID 28296572.
- ^ Buddeweg A, Sharma K, Urlaub H, Schmitz RA (March 2018). "41 affects ribosome binding sites within polycistronic mRNAs in Methanosarcina mazei Gö1". Molecular Microbiology. 107 (5): 595–609. doi:10.1111/mmi.13900. hdl:11858/00-001M-0000-002E-9BB8-7. PMID 29271512.
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