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N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase

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N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase
Identifiers
EC no.2.3.2.17
Databases
IntEnzIntEnz view
BRENDABRENDA entry
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MetaCycmetabolic pathway
PRIAMprofile
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NCBIproteins

N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase (EC 2.3.2.17, femA (gene)) is an enzyme with systematic name N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-ditrans,octacis-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase.[1][2][3][4] This enzyme catalyses the following chemical reaction

N-acetylmuramoyl-L-alanyl-D-isoglutaminyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-diphospho-ditrans,octacis-undecaprenyl-N-acetylglucosamine + 2 glycyl-tRNA N-acetylmuramoyl-L-alanyl-D-isoglutaminyl-L-lysyl-(N6-triglycyl)-D-alanyl-D-alanine-diphospho-ditrans,octacis-undecaprenyl-N-acetylglucosamine + 2 tRNA

This enzyme catalyses the successive transfer of two glycine moieties from charged tRNAs to N-acetylmuramoyl-L-alanyl-D-isoglutaminyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine.

References

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  1. ^ Berger-Bächi B, Barberis-Maino L, Strässle A, Kayser FH (October 1989). "FemA, a host-mediated factor essential for methicillin resistance in Staphylococcus aureus: molecular cloning and characterization". Molecular & General Genetics. 219 (1–2): 263–9. doi:10.1007/bf00261186. PMID 2559314.
  2. ^ Johnson S, Krüger D, Labischinski H (October 1995). "FemA of Staphylococcus aureus: isolation and immunodetection". FEMS Microbiology Letters. 132 (3): 221–8. doi:10.1111/j.1574-6968.1995.tb07837.x. PMID 7590176.
  3. ^ Benson TE, Prince DB, Mutchler VT, Curry KA, Ho AM, Sarver RW, Hagadorn JC, Choi GH, Garlick RL (August 2002). "X-ray crystal structure of Staphylococcus aureus FemA". Structure. 10 (8): 1107–15. doi:10.1016/s0969-2126(02)00807-9. PMID 12176388.
  4. ^ Schneider T, Senn MM, Berger-Bächi B, Tossi A, Sahl HG, Wiedemann I (July 2004). "In vitro assembly of a complete, pentaglycine interpeptide bridge containing cell wall precursor (lipid II-Gly5) of Staphylococcus aureus". Molecular Microbiology. 53 (2): 675–85. doi:10.1111/j.1365-2958.2004.04149.x. PMID 15228543.
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