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TRNA:m4X modification enzyme

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TRNA:m4X modification enzyme
Identifiers
EC no.2.1.1.225
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
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PMCarticles
PubMedarticles
NCBIproteins

TRNA:m4X modification enzyme (EC 2.1.1.225, TRM13, Trm13p, tRNA:Xm4 modification enzyme) is an enzyme with systematic name S-adenosyl-L-methionine:tRNAPro/His/Gly(GCC) (cytidine/adenosine4-2'-O)-methyltransferase.[1] This enzyme catalyses the following chemical reaction

(1) S-adenosyl-L-methionine + cytidine4 in tRNAPro S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
(2) S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC) S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
(3) S-adenosyl-L-methionine + adenosine4 in tRNAHis S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis

The enzyme from Saccharomyces cerevisiae 2'-O-methylates cytidine4 in tRNAPro and tRNAGly(GCC), and adenosine4 in tRNAHis.

References

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  1. ^ Wilkinson ML, Crary SM, Jackman JE, Grayhack EJ, Phizicky EM (March 2007). "The 2'-O-methyltransferase responsible for modification of yeast tRNA at position 4". RNA. 13 (3): 404–13. doi:10.1261/rna.399607. PMC 1800514. PMID 17242307.
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