Talk:3β-Hydroxysteroid dehydrogenase

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Merger proposal

It is proposed to combine pages devoted to 3 beta hydroxysteroid dehydrogenase into this page. This proposal pertains to the following pages: 3beta(or 20alpha)-hydroxysteroid dehydrogenase, 3beta-hydroxy-Delta5-steroid dehydrogenase, HSD3B2, HSD3B1, Delta 4-5 isomerase Skingski (talk) 19:06, 17 July 2009 (UTC)[reply]

Support (all concern the same family of enzymes):

3-beta-HSD (this article) – EC 1.1.1.145
3beta-hydroxy-Delta5-steroid dehydrogenase – EC 1.1.1.145
Delta 4-5 isomerase – ?

Oppose (different EC numbers and therefore different enzymes):

Oppose (specific isozymes, in any case, not practical to merge all these large PBB templates into the same article):

Cheers. Boghog2 (talk) 20:43, 17 July 2009 (UTC)[reply]

Agree on HSD3B1, 2 and 7. Your links also bring to light that Cholest-5-ene-3beta,7alpha-diol 3beta-dehydrogenase and HSD3B7 should be merged since they are the same enzyme.
3beta(or 20alpha)-hydroxysteroid dehydrogenase is the same enzyme as 3betaHSD and should be merged here. My reasoning is:

1 - The described enzymatic reaction relies on a 3betaHSD -- no 20alpha activity at all.

2 - 20alphaHSD is an entirely different enzyme from 3beta.

3 - My own NCBI and PubMed searches reveal no enzyme synonymous with 3beta or 20 alpha HSD nor the alternative names with 20 alpha in their title given on that page (one of which is not an enzyme name - "3beta,20alpha-hydroxy steroid") nor any enzyme with both activities.

4 - EC numbers refer to reactions not enzymes - 3betaHSDs are in that rare enzyme class that can catalyze reactions with a variety of steroid substrates and thus a variety of ECs should be assigned to each 3betaHSD. Why there is a 20alpha reference is incomprehensible to me.

Skingski (talk) 17:45, 20 July 2009 (UTC)[reply]

Thanks for the explanation. I agree that Cholest-5-ene-3beta,7alpha-diol 3beta-dehydrogenase and HSD3B7 should be merged so I went ahead and implemented this merger. While I was at it, I also replaced the {{enzyme references}}, {{enzyme links}}, and {{GO code links}} templates with the single {{enzyme}} template which removes redundancies and is more compact.

I am not so sure on merging 3beta(or 20alpha)-hydroxysteroid dehydrogenase. There are some publications (see PMID 6958329, PMID 3483295, and PMID 2605227) that claim that there is an enzyme that can be purified to homogeneity that has both 3 beta and 20 alpha steroid reductase activities. Boghog2 (talk) 20:26, 22 July 2009 (UTC)[reply]

Great on the HSD3B7 merger. On merging 3beta(or 20alpha)-hydroxysteroid dehydrogenase:

EC 1.1.1.210 (3beta-diol to DHT) is shown in PMID 2139411 and PMID 7958395 to be carried out by 3beta-HSD enzymes. Therefore, it can be cited on the 3-beta-HSD page.

One lab, Sweet’s, authored all 3 papers you cite. No one seems to have worked on it since, including Sweet, who never obtained sequence data. This indicates that no one in the field supports this conjecture. Sweet estimated the MW for the cow protein is 55 kDa and the sheep, 35 kDa, which suggests they are 2 different enzymes.

3 publications cite Sweet. PMID 8172618 calls the protein 20alpha-HSD. PMID 8280099 calls it a 3beta, 20alpha-HSD, but in the Introduction, par. 1, notes that HSDs can have multiple activites; e.g., human 17beta-HSD exhibits low level 20alpha-HSD activity (see also PMID 17257829, PMID 2983668 ) (note it is still called 17beta, not 17beta,20alpha). Since Sweet’s protein(s) exhibits a 2 to almost 4-fold preference for the 3beta reaction over 20alpha, the sheep protein could be 3beta-HSD and the cow, ???. Skingski (talk) 21:25, 23 July 2009 (UTC)[reply]

I agree that it difficult to classify the enzymes that Sweet has described. My suggestion is that since EC 1.1.1.210 is still recognized by the Enzyme Commission, that the 3beta(or 20alpha)-hydroxysteroid dehydrogenase article be maintained but trimmed and the reader directed to 3-beta-HSD (this article) and 20alpha-hydroxysteroid dehydrogenase (AKR1C1). This proposal would not be a merger, but close to it. If at some future date 1.1.1.210 is depreciated, then the 3beta(or 20alpha)-hydroxysteroid dehydrogenase article could be converted into a redirect. Does this sound reasonable? Boghog2 (talk) 18:14, 24 July 2009 (UTC)[reply]

OK. Perhaps an explanation should be on the 3beta(or 20alpha)-hydroxysteroid dehydrogenase page describing the conditions related to its description by Sweet et al. so the reader knows it is a putative protein at this time. If I have time, I might put this issue to the EC folks.Skingski (talk) 20:43, 24 July 2009 (UTC)[reply]

The content of Delta 4-5 isomerase has now been merged into this article and 3beta(or 20alpha)-hydroxysteroid dehydrogenase has been trimmed and now includes links to 3-beta-HSD and 20alpha-hydroxysteroid dehydrogenase. Cheers. Boghog2 (talk) 16:19, 25 July 2009 (UTC)[reply]

You did a really awesome job!!! One question I have is shouldn't the boxes on HSD3B1 and HSD3B2 be moved from here and put on their respective wiki pages?Skingski (talk) 15:55, 26 July 2009 (UTC)[reply]

Thanks and thank you for your insights into this family of enzymes and for helping to remove the redundancy in this confusing collection of Wikipedia articles. The {{protein}} boxes included on this page are more compact version of the {{GNF_Protein_box}} on the gene/protein specific articles. So including the protein boxes in the HSD3B1 and HSD3B2 articles would be redundant. I think the protein boxes on this page are appropriate since they give an overview of the human 3-beta-HSD isozymes as well as include links to the specific isozyme pages. There are many such family pages (see for example alcohol dehydrogenase, estrogen receptor, angiotensin receptor) which include compact protein box templates which contain links to the individual members of their respective families. Does this make sense? Boghog2 (talk) 16:42, 26 July 2009 (UTC)[reply]

Other pages such as 17Beta Hydroxysteroid dehydrogenase and sulfotransferase, admittedly, not in the best of condition, do not do this, but refer you to individual pages which do have their own boxes. It makes sense because the number of protein boxes you'd have to add to the sulfotransferase page especially would be impractical. I guess I'm thinking that by having the protein box exclusively on the gene page, you get a one-stop look at the protein rather than shuttling between 2 pages, wherease the 3beta page itself gives a genreal overview. I don't know the wiki rule on this, so whatever you decide is fine by me.Skingski (talk) 17:18, 26 July 2009 (UTC)[reply]

Introduction

The introduction currently says the same thing 3 different times in 3 different ways (e.g., it says it is responsible for progesterone production 3 times). Can we decide which way we need to say this? Skingski (talk) 21:41, 1 August 2018 (UTC)[reply]