Talk:Illumina dye sequencing

Molecular Biology: Genetics / MCB / COMPBIO

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Wiki Education Foundation-supported course assignment

This article was the subject of a Wiki Education Foundation-supported course assignment, between 12 May 2020 and 22 June 2020. Further details are available on the course page. Student editor(s): Brizileigh. Peer reviewers: Tanner Stenlund, Bucear.

Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 22:50, 17 January 2022 (UTC)[reply]

Paired sequencing

In "Sequencing by synthesis", if the reverse strands are "washed away", how is paired-end sequencing performed? --87.138.112.29 (talk) 05:50, 1 September 2018 (UTC)[reply]

It seems that after the forward strand is sequenced, a new reverse strand is made, and then sequenced. Gah4 (talk) 22:41, 19 February 2021 (UTC)[reply]

Comparison with other methods

Comparing Illumina method with Sanger's is perhaps a bit outdated. I do not think many people use Sanger any more. I think a comparison with long reads approaches such as PacBio or Nanopore would be more meaningful. The latter would also permit a comparison between massive centralised sequencing and portable on-site one. NicGambarde (talk) 08:06, 23 June 2021 (UTC)[reply]

Clonal Amplification Section?

The "Clonal Amplification" section seems to just repeat the bridge amplification section and then discuss error detection, which should be in the "Data Analysis" section... FoldedGenes (talk) 23:12, 6 November 2023 (UTC)[reply]