Talk:Sarocladium kiliense/Archive 1

Peer Review by Joanne (lorgecow)

• Leading section: this portion of your article is missing. A resource you can use is MOS:LEAD. It outlines the formatting and language you should use.

• Taxonomy: this is a good introduction to your fungus, as it clarifies the nomenclature of your species from previous naming.

• Morphology: try to organize your information from macroscopic to microscopic (or vice versa) so the reader does not get confused. Try to find more sources that describe the microscopic morphology of your fungus. We only have an idea of when the conidia look like.
  • According to Khan et al. (2011), “[…] A. kiliense form unicellular chlamydospores and adelophialides (reduced forms of phialides without a basal septum) in the vegetative or substrate hyphae (not in aerial hyphae) when grown on oatmeal agar at 24°C for about 2 weeks.”
  • Khosia and Gupta (2016) have some information about morphology in their article.

• Ecology and growth conditions: you mention that your fungus grows on MEA at 20°C. Is there enough evidence to say that your fungus is mesophilic? If so, then you can exclude information about the medium and incubation time. When you mention that is was found in certain regions, what materials was it found on? If it is soil, then specify this.
  • Fabiola et al., (2014) states that your fungus is found worldwide, but that statement is unfounded, as the article they cited (https://www.sciencedirect.com/science/article/pii/S0166061614600452) never mentions where in the world your fungus can be found.

• Pathogenicity: I think that this section is good. It has a lot of info for you to expand on in your final article.
  • According to Khan et al. (2011), in immunocompromised patients, your fungus “may cause disseminated infections involving multiple organs following fungemia. In one autopsy-proven study of invasive mold infections in cancer patients, 10% of the cases were caused by Acremonium spp.”
  • According to Khosia and Gupta (2016), your fungus is an “endophytic fungus associated with crops such as fennel, lettuce, chicory and celery”. Additionally, it can cause “opportunistic infections in both immune-competent as well as immune-compromised human patients”.

• Diagnosis and treatment: treatment should be lowercase according to Wikipedia standards. A link for “amphotericin B” can be included for further reading. More information can be included in this section. For example:
  • According to Khan et al. (2011),” Generally, the management of Acremonium peritonitis includes catheter removal and systemic antifungal therapy”.
  • According to Khan et al. (2011), fungi of the genus Acremonium look similar and are hard to tell apart microscopically, which causes difficulties in identifying the etiological agent.

• Metabolism: you mention that your fungus produces an antibiotic – you can elaborate that in its own subsection with supporting information. This fungus metabolizes many substances, but what is the significance/purpose of this?

• References: I like that you used a lot of books as your references (however that means I cannot look through your references).
  • There are a few extra punctuation marks here, so watch out for that when entering the form. An example is seen in your first reference where the date is written as “1937-“.
  • Additionally, 2 of your citations display an error for the value entered for the date.

Lorgecow (talk) 14:18, 27 October 2018 (UTC)

Elizanpa's peer review

Hi Grey1016,

• I like how organized your outline is and the amount of important information you gathered is amazing!

• I noticed that your lead section is missing. It's basically just a summary or introduction of your fungus, and it should be located on top of your taxobox

Taxonomy

• To help add more information and make the readers know more about your fungi, I suggest talking about the genus Sarocladium
• Since you mentioned about Acremonium kiliense, it would also be good to talk about the differences between the two aforementioned genuses (i.e morphology)
• Here is an article I found with some specific information that can help you: [[1]]

Morphology

• Good points !
• You mentioned that S. kiliense appears to form a conidia. I read your [Khan2011] (ref #3) article and they also mentioned that under the microscopic level, S. kiliense starts off initially as a mycelium AND THEN it forms a conidia. You may want to include this part.
• Noting your point about the change in colour in SDA, your [Summerbell2011] (ref #6) article briefly talks about the ability of Sarocladium species to produce melanin and change to another colour in SDA as well. There is also a specific information that may be a good addition to your Pathogenicity section

Ecology and growth conditions

• Since you mentioned that S. kiliense is a soil fungi, it would be good to elaborate further on whether its a saprotroph or not. Your (ref #7) has this information
• It might be better to concise the two points talking about where the fungus is found into one point

Pathogenicity

• I like that you mentioned the groups of individuals at risk and the fact that they're not only pathogenic to humans (good job mentioning the name of the algae and the specific plants!)

Diagnosis and treatment

• I noticed that you mentioned Amphotericin B as a treatment against this fungus, and that it is continued to be used despite reports of treatment failure. Maybe its a good idea to talk about why S. kiliense is resistant to antifungal therapy (ie. minimum inhibitory content). Your (ref #5) has this information
• It would also be good to mention all of 4 antifungal therapies tested against your fungus. (Ref #5) and (ref #7) talked about them
• I found an article that talks about the efficacy of each of these therapies [[2]]

Metabolism

• You might want to go into detail as to how the enzymes and substances are produced, the mechanism of degradation of polysaccharides and starch, and how they oxidize manganese
• You can also add information, if there is or if you can find any, about secondary metabolites

Good luck on your article ! I hope this would be of help

Elizanpa (talk) 22:48, 1 November 2018 (UTC)

Some suggestions

• do not use in line citations
• I made some language edits to your text which hopefully will help you make further modifications
• watch subject-verb agreement: e.g., have been cross referenced with... --> has been cross referenced with (but I don’t understand what you mean by “have been cross referenced with”)
• there are a few things you can link, e.g., Egypt, Sabouraud Dextrose Agar, etc.
• try to use common language instead of scientific jargon where this is possible, e.g., chromophilic
• what is “cylindrical to ellipsoidal at the tip”?
• good content

Medmyco (talk) 19:44, 17 November 2018 (UTC)