User:Gkaltam/sandbox

I am starting this sandbox page to help me practice wikipedia editing.

Five Pillars

Pillar 1

Wikipedia is an online encyclopedia and it is not to be used as an advertising platform. It should also not be used as a personal blog, dictionary, or book.

Pillar 2

Wikipedia has a neutral point of view, thus there should not be any bias or opinionated pieces on here.

Pillar 3

Wikipedia provides free content to everyone that uses it. Thus copyright laws must be followed, and absolutely no plagiarism is allowed. This is just like school. Facts should be sourced by certifiable resources.

Pillar 4

RESPECT! Editors on Wikipedia should be nice and polite to one another. Treat someone the way you want to be treated. If there is a problem, talk it out to reach a resolution.

Pillar 5

There are no set rules. Have the courage to edit articles and do not be afraid to make mistakes.

Summary of characteristics of target article

1. Information is accurate. There are no big "holes" in the information that need to be filled.

2. Information is cited correctly. Inline citations are present and in the references.

3. The article is written well with no grammatical errors or slang. In addition, it should not be opinionated. If there is a controversy regarding the topic, both sides should be represented fairly.

4. The article is focused on its topic and does not get too into detail.

5. The article has a good "flow" to it, and the order of the information should not confuse the readers.

6. If applicable, and article should have illustrations with good captions.

Y-STR profiling

In the field of forensic science, Y-short tandem repeat profiling, or Y-STR, is used to pull out male DNA from a mixture of DNA in sexual assault cases. In many sexual assault cases, the victim is female, and thus extracting DNA from the victim will have the victim's DNA as well as the perpetrator's. By using Y-STR profiling, the victim's DNA will not be included in the Y-STR profile since this technique only targets sites on the Y chromosome. Besides forensic sexual assault cases, Y-STR profiling may also be used for lineage testing or paternal testing.

AB5 Toxin

This may be a good article to use to tell readers about the function of the B subunit in AB5 toxin because it informs us on each of the subunits function. It also lists what it might do in a human body response.:

Wang, H; Paton, JC; Herdman, BP; Rogers, TJ; Beddoe, T; Paton, AW (2013 Mar). "The B subunit of an AB5 toxin produced by Salmonella enterica serovar Typhi up-regulates chemokines, cytokines, and adhesion molecules in human macrophage, colonic epithelial, and brain microvascular endothelial cell lines.". Infection and immunity 81 (3): 673–83. PMID 23250951.

Gkaltam (talk) 15:46, 4 March 2014 (UTC)

I agree. All that information can be seen just from the abstract so I'm sure its just packed with information, however for some reason it will not let me access the full article by the link provided. I'm going to search for the full article using an alternative method (Library resources) but if you have access to the full article do you think you could email me a copy of it please. It will be much appreciated.ReeseLanger (talk) 06:14, 5 March 2014 (UTC)

I believe this will be a good article to use for Mechanism of Action as well as maybe Examples from other species.The article tell of the mode of action of AB5 toxins. It also gives us some examples of bacteria that produce the toxin.

Paton, AW; Beddoe, T; Thorpe, CM;et al. AB5 subtilase cytotoxin inactivates the endoplasmic reticulum chaperone BiP. Nature 2006;443:548-52.17024087ReeseLanger (talk) 02:23, 12 March 2014 (UTC)

The article I just put under the list of references has a good structural ribbon image for Subtilase cytotoxin. Gkaltam (talk) 03:32, 12 March 2014 (UTC)

This article may shed some light on how the toxin binds to receptors in the cell. It may provide more information on the mechanism of these toxins:

Gallegos, K. M., Conrady, D. G., Karve, S. S., Gunasekera, T. S., Herr, A. B., & Weiss, A. A. (2012). Shiga Toxin Binding to Glycolipids and Glycans. Plos ONE, 7(2), 1-10. doi:10.1371/journal.pone.0030368 Gkaltam (talk) 03:40, 12 March 2014 (UTC)

The clathrin dependent article has a really good understanding of the SubAB mechanism. Gkaltam (talk) 23:33, 16 March 2014 (UTC)

Preliminary Outline

1. Introduction- Expand upon the information listed regarding AB5 toxins

2. Four families of AB5 toxins.

3. Structure- Somewhat in place but needs better organization and detail

3a. A subunit - image to be added

3b. B subunit - image to be added

4. Mechanism of Action- More detail will be added with references to make it reliable

5. List of AB5 toxins - Currently exists, more research to be done

6. Current uses in Drugs

Mmehta10 (talk) 21:55, 7 March 2014 (UTC)

List of References

• Beddoe, Travis; Paton, Adrienne W.; Le Nours, Jérôme; Rossjohn, Jamie; Paton, James C. (July 2010). "Structure, biological functions and applications of the AB5 toxins". Trends in Biochemical Sciences. 35 (7): 411–418. doi:10.1016/j.tibs.2010.02.003. PMC 2929601. PMID 20202851.
• Le Nours, Jérôme; Paton, Adrienne W.; Byres, Emma; Troy, Sally; Herdman, Brock P.; Johnson, Matthew D.; Paton, James C.; Rossjohn, Jamie; Beddoe, Travis (6 August 2013). "Structural Basis of Subtilase Cytotoxin SubAB Assembly". Journal of Biological Chemistry. 288 (38): 27505–27516. doi:10.1074/jbc.M113.462622. PMC 3779744. PMID 23921389.
• Chong, Damien C.; Paton, James C.; Thorpe, Cheleste M.; Paton, Adrienne W. (March 2008). "Clathrin-dependent trafficking of subtilase cytotoxin, a novel AB toxin that targets the endoplasmic reticulum chaperone BiP". Cellular Microbiology. 10 (3): 795–806. doi:10.1111/j.1462-5822.2007.01085.x. PMID 18042253.