User:Kaitlyncottrell/sandbox

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Preferential Motor Reinnervation (PMR) refers to the tendency of a regenerating axon in the Peripheral Nervous System (PNS) to reinnervate a motor pathway as opposed to a somatosensory pathway.[1][2][3] The role of PMR affects how nerves regenerate and reinnervate within the PNS after surgical procedures or traumatic injuries. It is important to understand in order to further develop axonal regrowth surgical techniques. Further research of preferential motor reinnervation will lead to overall understanding of peripheral nervous system function in the human body in regards to cell roles and abilities.

Summary[edit]

Motor vs sensory nerve reinnervation[edit]

Nervous System Organization - The Motor and Sensory Systems
Nervous System Organization - The Motor and Sensory Systems

When a group of nerves is cut, the peripheral nervous system has somewhat of an ability to regrow the cut nerves, depending on a number of factors. Motor axons preferentially regenerate down motor pathways, even when faced with options to go down cutaneous pathways, or after manipulation. This tendency is influenced by a number of factors in the PNS system, including the Schwann cells and trophic factors that influence the motor nerve to choose the motor pathway.[2][3][4] The different systems are illustrated in the image displayed on the right, and preferential motor reinnervation applies specifically to the peripheral nervous system, which is illustrated in the photos of the bottom of the system shown.

Regeneration vs reinnervation: what is the real difference?[edit]

When peripheral axons are severed, the severed distal part of the axon degenerates. The only remaining parts from the original nerve are the Schwann cells which myelinate the peripheral axons. The basal lamina components that the Schwann cells secrete help to guide regeneration. The more precisely the axon stump is able to regrow in its original path, the better the recovery of function - especially regarding fine touch and movements. The growth of the axon stump to its original target is regeneration.[5] Reinnervation on the other hand, is the recovery of function through reestablishing synaptic connections. Even when the axon has degenerated, the Schwann cells and acetylcholine receptors remain in place, allowing for the junction to establish the original synapses once the axon stump regenerates.[6] In the real world, though there is a defined difference in regeneration vs reinnervation, it is not commonly differentiated. Many professionals use the terms interchangeably. This is because without regeneration, there would not be a nerve to innervate, but without reinnervation, the nerve would not function. So because both are necessary before a severed nerve functions again, professionals do not commonly differentiate between the two terms.

Why is preferential motor reinnervation relevant?[edit]

Knowledge of preferential motor reinnervation is necessary because of how it affects the regeneration of nerves. When a patient has lost nerve function, the different attempts in regrowing the nerve and helping improve its function are very often interfered with by PMR effects. The more the medical world understands how a motor nerve will grow as a result of its preferential support from the motor pathways, the better the surgeons and nerve repair can be. The reinnervation is very affected by which pathway the regenerated nerve has gone down. The nerves ability to function properly after damage is very dependent on this outcome, which is why this is such a relevant topic. The affect of a nerve reinnervation after different grafting techniques is a very focused on topic currently, because through nerves are able to regenerate, they often target incorrectly and thus full recovery is not reached.[7][8]

How do nerves regrow?[edit]

Process of a cut nerve regenerating[edit]

A cut axon in the peripheral nervous system has two parts: a distal and a proximal axon stump. The space in between the two stumps is known as the gap, and is what the nerve must grow through in order to fully regenerate and reinnervate. The distal axon is degenerated through the body's own mechanisms, mostly macrophage consumption and enzymes breaking it down. The proximal part of the cut axon many times is able to regenerate.[5][9] The regeneration and reinnervation of the cut nerve is affected by multiple factors, including how far the nerve must regrow, what kind of environment it is growing in, and the different Schwann cells and pathway options available. PMR indicates that a regenerating motor neuron will choose a motor pathway Schwann cell over a cutaneous pathway Schwann cell when regenerating.[10][11]

The role of Schwann cells[edit]

Cultured Schwann cell

Schwann cells are the myelination cells that surround nerves. When multiple nerves are cut, they must regrow and enter back through one of the Schwann cells that makes up the distal stump of the gap. These Schwann cells support axonal regrowth through their production of trophic factors as well as surface expression of multiple cell adhesion molecules that help influence axonal growth.[4][12]

Neurotrophic support in reinnervation[edit]

Neurotrophic factors are support proteins and factors that help assist in the growth and maintenance of axons throughout the body. Different cells emanate different proteins, but the ones specific to the peripheral nervous system play a major role in regeneration of cut nerves in the peripheral nervous system.[13][14] In relation to reinnervation, neurotrophic support is key in assisting with supporting the regeneration of axons. Some discussion has led investigators to believe that neurotrophic factors only led to more axonal sprouting rather than actually influencing the regeneration. The ability of neurotrophic factors to influence the sprouting of axons has been seen with electron microscopic images and in multiple studies extensively detailed in a review of the role of neurotrophic factors in regeneration. In addition to the ability of the factors to influence sprouting, Schwann cells in particular show a significant upregulation of a number of trophic factors after undergoing axotomy.[12][14] One major difference in motor and sensory pathways is the difference in what trophic factors are upregulated by the Schwann cells of those pathways. Denervenated motor Schwann cells upregulate BNDF and p75, whereas sensory pathway Schwann cells upregulate a number of other varied trophic factors. This difference in trophic factor support is suspected to be a major influencer of preferential motor reinnervation.[14][12] Though it is a major factor, inherent molecular differences do not alone determine the reinnervation pathway of the motor neurons[15], as demonstrated in a study done in a mouse femoral nerve, where the size of the pathways were manipulated, leading to incorrect motor axon pathway reinnervation.[16]

Preferential motor reinnervation influencing factors[edit]

End organ contact: muscle vs. skin[edit]

Reinnervation specificity is also influenced by the end-organ contact of the different pathway options for the axon. End organ contact is statistically insignificant after two weeks, which is when end-plate reinnervation typically is just starting. However, after that time period, end-organ contact statistically influences the reinnervation ability of axon projection. When the end of the pathway is a muscle contact area, there is a significant difference in the number of motor neurons reinnervating. [2][15]

Cellular & molecular mechanisms that play a key role in reinnervation[edit]

These are trophic factors that are discussed in detail in above sections. These factors can influence where an axon grows towards, mostly from chemotaxis affects that the different proteins have on the growing axon's directionality. The trophic factors differ between motor and sensory pathways, which is a major influential factor in preferential motor reinnervation.[12][14][17]

Size of the terminal nerve branch[edit]

The terminal nerve branch size has a lot of influence on the reinnervation pathway of the axon. When two pathways, one cutaneous and one motor, are roughly comparable in size, the motor axons follow preferential reinnervation patterns along the motor pathways. However, enlargement of sensory pathways in the same experiment led to the motor axons to reinnervate those pathways, indicating that trophic factors alone do not cause reinnervation of motor neurons. This is shown because the motoneurons wrongly reinnervate down pathways that are sensory, thus demonstrating that the size of the terminal nerve branch pathway can affect the axonal reinnervation patterns.[16]

Reinnervation accuracy[edit]

Whether or not a growing axon is able to accurately reach the correct Schwann cell and eventually site of innervation has a large influence on PMR. The specificity of a motor axon to preferentially choose the motor pathway is the very essence of preferential motor reinnervation. Additionally, it influences whether or not a nerve can truly experience full reinnervation and recovery of function that is likened to what it had before the injury. Thus, this accuracy has everything to do with influencing whether or not a motor axon indicates preferential motor reinnervation. Different studies are investigating how an axon pathway specificity can be manipulated in order to see what kind of surgical advances can be made in regards to neuron repair.[1][15]

Current research: how is knowledge from studies being used in medicine?[edit]

The varied accuracy of damaged axons regenerating and reaching their original target end is a large reason that functional recovery of damaged nerves is such a variable in the peripheral nervous system.[10] The understanding of what Schwann Cell tube axons tend to reinnervate has implications for if a nerve will be able to become functional again after damage. If the axon is a subcutaneous axon and ends up in a motor Schwann Cell tube, it will not be able to innervate the muscle it ends up connected to. Thus, understanding how axons do reinnervate, and how motor axons can be pushed towards the correct regeneration site is an area of study that is extremely beneficial in helping to advance nerve repair in the PNS system. In 2004, a study looked at how Lewis rats' sensory vs motor nerve grafts affected the regeneration of a cut mixed nerve system (both motor and sensory nerves). It was noted that after 3 weeks, a mixed nerve defect had undergone substantial regeneration when paired with a motor nerve graft or a mixed nerve graft. In comparison, a sensory nerve graft was statistically less effective in regeneration, looking specifically at nerve fiber count, percent nerve, and nerve densities as the main three comparisons between the different grafts. This means that the best surgical practices in regenerating nerves in regards to PMR is using a nerve graft that is either a motor or a combination nerve graft.[18] In a study published in 2009, the terminal nerve branch size was investigated to see how it affected nerve regeneration. It was discovered that the branches of similar size initially regenerated about equally between cutaneous and muscular pathways, but after a while favored muscle branch paths. The study end results predicted that axonal collateral formation at the injured site being increased could increase regeneration accuracy. Understanding PMR affects would help overall in gaining a better understanding of the forces that influence the neuron repair, which was the overall conclusion of what was needed to help nerves functionally repair. This increasing understanding will overall impact surgical and repair processes with peripheral nerve repair. Though manipulation of axonal collateral formation may help, the further understanding of PMR will allow for the surgical practices and medical advances in nerve repair to continue developing.[16][15]

References[edit]

  1. ^ a b Robinson, Grant (2005). "Manipulations of the Mouse Femoral Nerve Influence the Accuracy of Pathway Reinnervation by Motor Neurons". Experimental Neurology. 192 (1): 39–45. doi:10.1016/j.expneurol.2004.10.013. PMID 15698617. S2CID 41726390. {{cite journal}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)
  2. ^ a b c Brushart, M. E. (1993). Motor Axons Preferentially Reinnervate, 13(June), 2730–2738.
  3. ^ a b Madison, R. D., Archibald, S. J., Lacin, R., & Krarup, C. (1999). Factors contributing to preferential motor reinnervation in the primate peripheral nervous system. The Journal of neuroscience : the official journal of the Society for Neuroscience, 19(24), 11007–16. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10594081
  4. ^ a b Bunge, R. P. (1994). The role of the Schwann cell in trophic support and regeneration. Journal of neurology, 242(1 Suppl 1), S19–21. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/7699403
  5. ^ a b Purves, Dale, George Augustine, et al. "Repair and Regeneration in the Nervous System." Neuroscience. Pages 563-567. Sunderland, MA
  6. ^ Purves, Dale, George Augustine, et al. "Repair and Regeneration in the Nervous System." Neuroscience. Pages 567-569. Sunderland, MA
  7. ^ Franz, C. K., Rutishauser, U., & Rafuse, V. F. (2008). Intrinsic neuronal properties control selective targeting of regenerating motoneurons. Brain : a journal of neurology, 131(Pt 6), 1492–505. doi:10.1093/brain/awn039
  8. ^ Hsieh, J.-H., Lin, W.-M., Chiang, H., Chang, L.-Y., Wu, C.-T., Pu, C.-M., … Hsieh, S.-T. (2013). Patterns of target tissue reinnervation and trophic factor expression after nerve grafting. Plastic and reconstructive surgery, 131(5), 989–1000. doi:10.1097/PRS.0b013e3182870445
  9. ^ Daly, W., Yao, L., Zeugolis, D., Windebank, a, & Pandit, a. (2012). A biomaterials approach to peripheral nerve regeneration: bridging the peripheral nerve gap and enhancing functional recovery. Journal of the Royal Society, Interface / the Royal Society, 9(67), 202–21. doi:10.1098/rsif.2011.0438
  10. ^ a b Robinson, Grant (2004). "Motor Neurons Can Preferentially Reinnervate Cutaneous Pathways". Experimental Neurology. 190 (2): 407–413. doi:10.1016/j.expneurol.2004.08.007. PMID 15530879. S2CID 26068046. {{cite journal}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)
  11. ^ Abdullah, M., O’Daly, a, Vyas, a, Rohde, C., & Brushart, T. M. (2013). Adult motor axons preferentially reinnervate predegenerated muscle nerve. Experimental neurology, 249C, 1–7. doi:10.1016/j.expneurol.2013.07.019
  12. ^ a b c d Höke, a, Redett, R., Hameed, H., Jari, R., Zhou, C., Li, Z. B., … Brushart, T. M. (2006). Schwann cells express motor and sensory phenotypes that regulate axon regeneration. The Journal of neuroscience : the official journal of the Society for Neuroscience, 26(38), 9646–55. doi:10.1523/JNEUROSCI.1620-06.2006
  13. ^ Deister, C., & Schmidt, C. E. (2006). Optimizing neurotrophic factor combinations for neurite outgrowth. Journal of neural engineering, 3(2), 172–9. doi:10.1088/1741-2560/3/2/011
  14. ^ a b c d Localization, S. (2009). The role of neurotrophic factors in nerve regeneration, 26(February), 1–10. doi:10.3171/FOC.2009.26.2.E3
  15. ^ a b c d Madison, R. D., Robinson, G. a, & Chadaram, S. R. (2007). The specificity of motor neurone regeneration (preferential reinnervation). Acta physiologica (Oxford, England), 189(2), 201–6. doi:10.1111/j.1748-1716.2006.01657.x
  16. ^ a b c Robinson, G. a, & Madison, R. D. (2009). Influence of terminal nerve branch size on motor neuron regeneration accuracy. Experimental neurology, 215(2), 228–35. doi:10.1016/j.expneurol.2008.10.002
  17. ^ Martini, R. (1994). Expression and functional roles of neural cell surface molecules and extracellular matrix components during development and regeneration of peripheral nerves. Journal of neurocytology, 23(1), 1–28. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/8176415
  18. ^ Nichols, C. M., Brenner, M. J., Fox, I. K., Tung, T. H., Hunter, D. a, Rickman, S. R., & Mackinnon, S. E. (2004). Effects of motor versus sensory nerve grafts on peripheral nerve regeneration. Experimental neurology, 190(2), 347–55. doi:10.1016/j.expneurol.2004.08.003

External links[edit]

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