User:Michelle Meyer BC/L20
| Ribosomal protein L20 leader | |
|---|---|
 Predicted secondary structure and sequence conservation of L20_leader | |
| Identifiers | |
| Symbol | L20_leader |
| Rfam | RF00558 |
| Other data | |
| RNA type | Cis-reg; leader |
| Domain(s) | Bacteria |
| SO | SO:0000233 |
| PDB structures | PDBe |
The structure of this family of ribosomal regulatory protein leaders was discovered independently both computationally[1] and experimentally[2] at the same time. Unlike its apparently non-homologous counterpart in Gammaproteobacteria, the Firmicute L20-interacting RNA regulates at the level of transcription, not translation [3] The RNA structure is located in the 5' untranslated regions of mRNAs encoding initiation factor 3 followed by ribosomal proteins L35 and L20 (infC-rpmI-rplT). [2] The Firmicute L20-interacting regulatory RNA consists of a 3-stem-loop structure, including a terminator stem. An additional non-binding stem was reported [2], but was only variably present and therefore not included in the consensus structure. Ribosomal protein L20 binds this leader sequence between the first two helices, and it is believed to function via a molecular mimicry model, due to its resemblance to the L20 binding site on the 23S rRNA. [2]
The L20-binding RNA is most notably found in the Firmicute classes Bacilli and, to a lesser degree, Clostridia. Its presence in Thermotogae and Actinobacteria suggests possible horizontal transfer events.
References
[edit]- ^ Yao, Zizhen; Barrick, Jeffrey; Weinberg, Zasha; Neph, Shane; Breaker, Ronald; Tompa, Martin; Ruzzo, Walter L. (2007). "A computational pipeline for high- throughput discovery of cis-regulatory noncoding RNA in prokaryotes". PLOS Comput Biol. 3 (7): e126. doi:10.1371/journal.pcbi.0030126. PMC 1913097. PMID 17616982.
{{cite journal}}: CS1 maint: date and year (link) CS1 maint: unflagged free DOI (link) - ^ a b c d Choonee, N.; Even, S.; Zig, L.; Putzer, H. (2007). "Ribosomal protein L20 controls expression of the Bacillus subtilis infC operon via a transcription attenuation mechanism". Nucleic Acids Res. 35 (5): 1578–1588. doi:10.1093/nar/gkm011. PMC 1865079. PMID 17289755.
{{cite journal}}: CS1 maint: date and year (link) - ^ Guillier, M.; Allemand, F.; Raibaud, S.; Dardel, F.; Springer, M.; Chiaruttini, C. (2002). "Translational feedback regulation of the gene for L35 in Escherichia coli requires binding of ribosomal protein L20 to two sites in its leader mRNA: a possible case of ribosomal RNA-messenger RNA molecular mimicry". RNA. 8 (7): 878–889. doi:10.1017/s1355838202029084. PMC 1370305. PMID 12166643.
{{cite journal}}: CS1 maint: date and year (link)
External links
[edit]Category:Ribosomal protein leader
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