User:Patelurology2/Metabolic syndrome, hypertension, dementia, drug therapy, interactions, side effects, pathophysiology
Patelurology2/Metabolic syndrome, hypertension, dementia, drug therapy, interactions, side effects, pathophysiology[edit]
Formation of Beta Amyloid[edit]
Aβ is formed after sequential cleavage of the amyloid precursor protein, a transmembrane glycoprotein of undetermined function. APP can be processed by α-, β- and γ-secretases; Aβ protein is generated by successive action of the β and γ secretases. The γ secretase, which produces the C-terminal end of the Aβ peptide, cleaves within the transmembrane region of APP and can generate a number of isoforms of 39-43 amino acid residues in length. The most common isoforms are Aβ40 and Aβ42; the shorter form is typically produced by cleavage that occurs in the endoplasmic reticulum, while the longer form is produced by cleavage in the trans-Golgi network.[1] The Aβ40 form is the more common of the two, but Aβ42 is the more fibrillogenic and is thus associated with disease states. Mutations in APP associated with early-onset Alzheimer's have been noted to increase the relative production of Aβ42, and thus one suggested avenue of Alzheimer's therapy involves modulating the activity of β and γ secretases to produce mainly Aβ40.[2]
- [1] Clearance of amyloid-beta in Alzheimer’s
disease: progress, problems and perspectives
Metabolism & Transport of β Amyloid fragments[edit]
Potential Role of Endogenous and Exogenous Ab Binding Molecules in Ab Clearance and Metabolism
- Study indicates that intrasynaptic oAβ42, but not oAβ40, acutely inhibits transmission at the squid giant synapse. This inhibition is molecularly tied to a cascade of events involving CK2 activation and the rapid clathrin-independent endocytosis pathway. The reduction of FAT induced by oAβ42 showed in the accompanying article, in combination with our results showing a dramatic acute inhibition of synaptic transmission after intrasynaptic injection of oAβ42, represent novel findings concerning AD synaptic failure now clearly associated with a reduction of synaptic vesicle pools and transmitter release.
- Synaptic transmission block by presynaptic injection of oligomeric amyloid beta - oAβ42, but not oAβ40 or extracellular oAβ42
- Effective therapeutic intervention in progressive neurological disorders depends on a clear understanding of the molecular mechanisms associated with the disease in question. In this manuscript we have shown that dysregulation of CK2 by oAβ is capable of inhibiting the vital neuronal process of FAT. Therefore, we propose that pharmacological regulation of CK2 activity represents a promising target for therapeutic intervention in AD, particularly when combined with treatments that help manage GSK3 activity as well.Disruption of fast axonal transport is a pathogenic mechanism for intraneuronal amyloid beta
ACE Inhibitors[edit]
Examples=
ACE inhibitors can be divided into three groups based on their molecular structure:
Sulfhydryl-containing agents[edit]
- Captopril (trade name Capoten), the first ACE inhibitor
- Zofenopril
Dicarboxylate-containing agents[edit]
This is the largest group, including:
- Enalapril (Vasotec/Renitec)
- Ramipril (Altace/Tritace/Ramace/Ramiwin)
- Quinapril (Accupril)
- Perindopril (Coversyl/Aceon)
- Lisinopril (Lisodur/Lopril/Novatec/Prinivil/Zestril)
- Benazepril (Lotensin)
Phosphonate-containing agents[edit]
- Fosinopril (Monopril) is the only member of this group
Naturally occurring[edit]
Casokinins and lactokinins are breakdown products of casein and whey that occur naturally after ingestion of milk products, especially cultured milk. Their role in blood pressure control is uncertain.[3] The tripeptides Val-Pro-Pro and Ile-Pro-Pro produced by the probiotic Lactobacillus helveticus have been shown to have ACE-inhibiting and antihypertensive functions.[4]
Blood Brain Barrier, ACE & ACE Inhibitors, Dementia[edit]
Further, functionally can be grouped according to ability to cross Blood Brain Barrier- implication currently being studied for ability to affect dementia. List:
BBB Crossing ACE
- Lisinopril
- Perindropril
- Ramipril
- Trandolapril
- Captopril
- Fosinopril
BBB Non Crossing ACE
- Benzapril
- Enalapril
- Moexipril
- Imidapril
Other Antihypertensives, and even all ACE Inhibitors, possibly indirectly via RAAS feedback some of the effects on dementia and related complex - Construct: deemntia and BP dynamics and possibly incorporating known circadian rythm affecting Beta amyloid.
Choosing an ACE Inhibitor[edit]
Several ACE inhibitors are on the market. Here is a list of some by generic name followed by brand name(s).
- benazepril (Lotensin)
- captopril (Capoten)
- enalapril (Lexxel, Vaseretic, Vasotec)
- fosinopril (Monopril)
- lisinopril (Prinivil, Prinzide, Zestoretic, Zestril)
- moexipril (Univasc)
- quinapril (Accupril)
- ramipril (Altace)
- trandolapril (Mavik, Tarka)
- .Even hydrophilic ACE inhibitors can result in marked inhibition of brain ACE inside the BBB but that different brain structures show variable inhibition
- Perindopril improved learning and memory in AD rats, which correlated with decreased ACE activity and delayed AD
- BBB crossing ACE inhibitors/Centrally active ACE inhibitors include
- captopril (Capoten, Bristol-Myers Squibb)
- fosinopril (Monopril, Bristol-Myers Squibb)
- ramipril (Altace, King Pharmaceuticals)
- trandolapril (Mavik, Abbott Laboratories, Tarka)
- lisinopril (Prinivil, Prinzide, Zestoretic, Zestril)
- Perindropril
- BBB Non Crossing/ Non–centrally active ACE inhibitors include
- benazepril (Lotensin, Novartis Pharmaceuticals)
- enalapril (Vasotec, Merck ,Lexxel )
- moexipril (Univasc, Schwarz Pharma)
- quinapril (Accupril, Pfizer)
- Imidapril
Benefical Role of Centrally Acting ACE Inhibitors in Congestive Heart Failure[edit]
- Benefical Role of Centrally Acting ACE Inhibitors in Congestive Heart Failure
- Critically important contribution of the brain renin-angiotensin system to the pathophysiology of congestive heart failure.
- ALDO of adrenal origin enters the hypothalamus in direct proportion to plasma levels and suggest that ALDO contributes to the upregulation of hypothalamic RAS activity and sympathetic drive in heart failure.
- Sympathetic neuronal regulation of the heart in aging and heart failure Sympathetic nervous system in the failing heart and the healthy, aging heart, and consider whether the sympathetic activation accompanying aging may, perhaps, underlie and contribute to the neural pathophysiology of heart failure.... conclusion , on balance, that this proposition is not supported by the available evidence.
Dynamics & Flux Between Amyloid β fragments Beta amyloid & Blood Pressure & Autonomous System- Dementia as a Case Study[edit]
- Brain has ACE enzyme which takes part in local RAAS and converts Aβ42 ( plaquogenic ) to Aβ40( more soluble and removal ) forms of Beta amyloid ; latter is prdominentally a function of N domain portion on the ACE enzyme; Inhibition of ACE with ACE Inhibitors, especially Blood Brain Barrier crossing and with preferentially select N terminal activity would cause accumulation of Aβ42 which is plaquogenic causing progression of dementia; preferential C domain active BBB crossing ACE would linkely have less of this latter effect.
- ACE inhibitors vs ACE for and against dementia
- Whether plaque is dementic environment or the downstream small fragment generated by the action of ACE are inflammatory and hence dementic an environment, is the question!
Ach dynamics and Dementia[edit]
Angiotensin II Inhibitors and Dementia[edit]
Calcium Channel Blockers and Dementia[edit]
Central ACE & ACE Inhibitors[edit]
- Mass spectral fragmentation reactions of angiotensin-converting enzyme (ACE) inhibitors
- Mass spectral fragmentation reactions of angiotensin-converting enzyme (ACE) inhibitors: n interesting dissociation process (rearrangement) unique to one of the compounds, lisinopril, has been investigated using isotopic labeling experiments and exact mass measurements. The general nature of the process has been probed through both the positive and negative ion analyses of fourteen related compounds exhibiting structural homolog
Wondering : ACE ARBS BP Statins : Interactions and complexities[edit]
- Interactions and complexities
- Angiotensin-converting enzyme in renal and cerebral tissue and implications for successful blood pressure management.
-
- Plasma and tissue ACE Activity and ACE Inhibitors e.g. Quinapril tissue ACE -aorta
- The renin-angiotensin system in the heart and vascular wall: new therapeutic aspects.1994 a deletion polymorphism in the gene encoding ACE is a risk factor in myocardial infarction (MI)
- Anti-ischemic potential of drugs related to the renin-angiotensin system.
- Anti-ischemic potential of drugs related to the renin-angiotensin system.
- Bradykinin-mediated cardiovascular protective actions of ACE inhibitors. A new dimension in anti-ischaemic therapy?
- Bradykinin-mediated cardiovascular protective actions of ACE inhibitors. A new dimension in anti-ischaemic therapy?
- The sympathetic nervous system and ischaemic heart disease.
- The sympathetic nervous system and ischaemic heart disease.
- Effect of ACE inhibition on neurohormones.
- Effect of ACE inhibition on neurohormones.
- Effect of ACE inhibition on myocardial ischaemia.
- Effect of ACE inhibition on myocardial ischaemia. ACE inhibition improves endothelial function, exerts anti-atherogenic and anti-proliferation activity and modulates sympathetic activity.
- Vascular protective effects of angiotensin converting enzyme inhibitors and their relation to clinical events.
- Vascular protective effects of angiotensin converting enzyme inhibitors and their relation to clinical events. ACE inhibitors augment endothelium-dependent relaxation to bradykinin, while those to acetylcholine remain unaffected, at least in the time frame of the published studies, i.e. 3-6 months. In patients with coronary artery disease, however, paradoxical vasoconstriction to acetylcholine is markedly reduced after 6 months of ACE inhibition. After myocardial infarction ACE inhibitors reduce the development of overt heart failure, the occurrence of reinfarction and cardiovascular death in hypertensive patients. These effects have also been demonstrated in a subgroup analysis of the SOLVD (Studies of Left Ventricular Dysfunction) trial. Thus, in summary, ACE inhibitors are an important class of drugs providing cardiovascular protection in patients with increased cardiovascular risk.
BBB Crossing Statins and effecton brain, RAAS ACE ARBS Dementia[edit]
- Lipophilic but not hydrophilic statins selectively induce cell death in gynecological cancers expressing high levels of HMGCoA reductase. apoptotic potential of two lipophilic statins (lovastatin and simvastatin) and one hydrophilic statin (pravastatin) was assessed in cancer cell lines (ovarian, endometrial, and cervical) and primary cultured cancerous and normal tissues.
- Amyloid beta toxicity dependent upon endothelial cell state. ? statins may helpbesides conventional endothelial effect
- Differential-Effects-of-Statins-and-Alendronate-on-Cholinesterases
- Statins Show Promise in Protecting Against Alzheimer's! -statins might be able to protect against Alzheimer's earlier in the disease process in persons who do not yet have any cognitive impairment
- Side Effects and Dangers of Statin Drugs
- Which Statin Should I Take?
- Statins Prevent You from Getting Dementia?-5-year follow-up, persons who had used statins were about half as likely as those who did not use statins to develop dementia. experts suggest that brain permeant (crossing blood-brain-barrier) statins (such as simvastatin) might be far more effective than statins that don't get into the brain (atorvastatin). People should take statins only for purposes they are originally indicated for, that is: for cholesterol-lowering.
BLOOD-BRAIN BARRIER DRUG TARGETING: THE FUTURE OF BRAIN DRUG DEVELOPMENT[edit]
BLOOD-BRAIN BARRIER DRUG TARGETING: THE FUTURE OF BRAIN DRUG DEVELOPMENT
BBB Crossing & non Crossing ARB(s) and effect on brain, RAAS & Dementia[edit]
- Franz Volhard Lecture. Renin-angiotensin system: a dual tissue and hormonal system for cardiovascular control
- Clinical Profile of Eprosartan: A Different Angiotensin II Receptor Blocker-only that crosses BBB
BBB Crossing ACE Inhibitors, though Amyloid-Plaquogenic, reported Preventing Progress of Dementia: Conundum Redux[edit]
- Omapatrilat could have some advantages over lisinopril in the treatment of patients with congestive heart failure.
- Omapatrilat could have some advantages over lisinopril in the treatment of patients with congestive heart failure.
- Most of the ACE inhibitors on the market today are non-selective towards the two active sites of ACE because their binding to the enzyme is based mostly on the strong interaction between the zinc atom in the enzyme and the strong chelating group on the inhibitor. The resolution of the 3D structure of germinal ACE, which has only one active site that corresponds with C-domain of the somatic ACE, offers a structural framework for structure-based design approach. Although N- and C-domain have comparable rates in vitro of ACE hydrolyzing, it seems like that in vivo the C-domain is mainly responsible for regulating blood pressure. This indicates that C-domain selective inhibitors could have similar profile to that of a current non-selective inhibitors. Angiotensin I is mainly hydrolyzed by the C-domain in vivo but bradykinin is hydrolyzed by both active sites. Thus, by developing a C-domain selective inhibitor would permit some degradation of bradykinin by the N-domain and this degradation could be enough to prevent accumulation of excess bradykinin which has been observed during attacks of angioedema. C-domain selective inhibition could possibly result in specialized control of blood pressure with less vasodilator-related adverse effects. N-domain selective inhibitors on the other hand give the possibility of opening up novel therapeutic areas. Apparently, the N-domain doesn’t have a big role in controlling blood pressure but it seems to be the principal metabolizing enzyme for AcSDKP, a natural haemoregulatory hormone.
- In Vitro and In Vivo Inhibition of the 2 Active Sites of ACE by Omapatrilat, a Vasopeptidase Inhibitor
- The vasopeptidase inhibitor omapatrilat inhibits both neutral endopeptidase and angiotensin-converting enzyme (ACE). The in vitro and in vivo inhibitory potency of omapatrilat and the specific ACE inhibitor fosinopril toward the 2 active sites of ACE (called N- and C-domains) was investigated with the use of 3 substrates: angiotensin I, which is equally cleaved by the 2 ACE domains; hippuryl-histidyl-leucine, specific synthetic substrate of the C-domain in high- salt conditions; and a newly synthesized specific substrate of the N-domain designed by acetylating the lysine residue of AcSDKP. In vitro, omapatrilat was 5 times more potent than fosinoprilat in inhibiting angiotensin I hydrolysis. Omapatrilat inhibited similarly both N- and C-domain hydrolysis, whereas fosinoprilat was slightly more specific for the N-domain. The in vivo selective inhibitory potency of single oral doses of 10 mg omapatrilat and 20 mg fosinopril were investigated in a double-blind, placebo-controlled, cross-over study in 9 mildly sodium-depleted normotensive subjects. In accordance with the in vitro results, fosinopril appeared to be more specific for the N-domain than the C-domain in vivo, since plasma and urine AcSDKP concentrations were significantly higher than those observed with omapatrilat. This study shows that it is possible to assess separately in vitro and in vivo the selectivity of ACE or ACE/neutral endopeptidase inhibitors. A differential selectivity may explain some peculiar properties observed with some ACE inhibitors.
- Angiotensin converting enzyme domain structure and properties
- Structure of angiotensin I-converting enzyme. The inhibitor complex does provide insights into the network of hydrogen-bonding and ionic interactions in the active site as well as the mechanism of ACE substrate hydrolysis. The three-dimensional structure of ACE now paves the way for the rational design of a new generation of domain-selective ACE inhibitors.
- Abeta40 protects non-toxic Abeta42 monomer from aggregation.
- The N-terminal active centre of human angiotensin-converting enzyme degrades Alzheimer amyloid beta-peptide.
- ACE N & C domains in competition
- Potency For C domain L > E > C Lisinopril, Enalprilat, Captopril
- Potency For N domain C > E > L
- Aß42-40 converting inhibition is solely in N domain where Captopril is the strongest N: C>E>L
- ACE Inhibiting activity is solely in C domain where.........Lisionopril is the strongest C: L>E>C
Aβ42-to-Aβ40-converting activity is solely found in the N-domain of ACE and the angiotensin-converting activity is found predominantly in the C-domain of ACE. The N-linked glycosylation is essential for both Aβ42-to-Aβ40- and angiotensin-converting activities and that unglycosylated ACE rapidly degraded. The domain-specific converting activity of ACE suggests that ACE inhibitors could be designed to specifically target the angiotensin-converting C-domain, without inhibiting the Aβ42-to-Aβ40-converting activity of ACE or increasing neurotoxic Aβ42.
- ACE angiotensin-converting enzyme
- Aβ amyloid β-protein
- F-ACE full-domain ACE
- N-ACE N-terminal domain ACE
- C-ACE C-terminal domain ACE
- ACE N & C domains in competition
- While ACE inhibitors as a class do not appear to be independently associated with dementia risk or cognitive decline in older hypertensive adults, there may be within-class differences in regard to these outcomes. These results should be confirmed with a randomized clinical trial of a centrally active ACE inhibitor in the prevention of cognitive decline and dementia... no washout
- Aß42–40 conversion 120 links
- Angiotensin-Converting Enzyme Converts Amyloid ß-Protein 1–42 (Aß1–42) to Aß1–40, and Its Inhibition Enhances Brain Aß Deposition
- The upregulation of ACE activity can be a novel therapeutic strategy for AD.
- ACE inhibitors could be designed to specifically target the angiotensin-converting C-domain, without inhibiting the Aβ42-to-Aβ40-converting activity of ACE or increasing neurotoxic Aβ42
Centrally Active ACE Inhibitors May Help Prevent Dementia -coming soon -! can? non central ACE & possibly other Non ACE antihypertensives, be more preventive? Why recent study finds otherwise? FACTORS IN CONSTRUCT. ACE Degrades/converts Amyloid Aβ42 ( fibrillogenic, plaque forming ! ) to favorable Aβ40 more soluble possibly 'removable' amyloid & Central acting (BBB crossing) ACE Inhibitors thus likely to increase dementia; anti-inflammatory effects of ACE Inhibitors likely to decrease dementia, Acute effect of ACE on Ach likely to increase short term acuity confusing the picture for evaluation
The following Construct development in progress and involve all these and more points in hypothesis development.
- Centrally acting ACE Inhibitors have acute Ach effects helping in mental acuity short term during which measurement may be biased and the long term effect of amyloid Aβ42 accumulation may be masked in testing; favorable anti-inflammatory effect not withstanding, long term more Aβ42 is deposited or not converted to more soluble, disposable or removable Aβ40. Vascular Aβ42 vs Aβ40 to be considered and could be important along with the favorable anti-inflammatory effect.
- Acute, Chronic and Chronic with short term non use ( washed off ) use of ACE considering short term acuity enhancing effects of Ach.
- Difference between Aβ42 ? more in nerves and ? Aβ40 more in vessel
- Aβ42 vs Aβ40
- Pleomorphism and genetics of ACE
- RAAS -Systemic vs Local and interactions and cross influence
- ACE Inhibitors: BBB crossing vs Non crossing
- Non-BBB crossing ACE negative effect studies ie if increased dementia, then..
- Other enzyme systems besides ACE
ALL above to be considered in both below: along with congruency of the two studies with each other and restrospective congruency with prior knowledge base of studies and general understanding of the science.
- Currently reported study in Archives of Intl Med July 09-BBB crossing ACE reduce Dementia needs explanation.... Standby
- Current AII AT1 Inhibitors -Sartan--retrospective VA study expl and corroboration standby......
- 2013 find needs to be processed: [http://archinte.jamanetwork.com/article.aspx?articleid=415176 Angiotensin-Converting Enzyme Inhibitors and Cognitive Decline in Older Adults With Hypertension
Results From the Cardiovascular Health Study: While ACE inhibitors as a class do not appear to be independently associated with dementia risk or cognitive decline in older hypertensive adults, there may be within-class differences in regard to these outcomes. These results should be confirmed with a randomized clinical trial of a centrally active ACE inhibitor in the prevention of cognitive decline and dementia.]
Alzheimer's-beta amyloid, tau protein,-- Drug Rember, Methylene Blue[edit]
Alzheimer's researchers are divided on whether the disease is caused by 'beta amyloid' (a peptide found in Alzheimer brains) or by 'tau protein' (normally used for cellular scaffolding, but can aggregate out of control and destroy neurons). Today in Chicago a new drug has been announced that stops tau aggregation and appears to have halted Alzheimer's-related decline in 300 clinical trial patients. The drug is known as 'rember.
Angiotensin Receptor Blockers lower progression of Alzheimer's Disease- ! explanation coming and Construct intergration in progress[edit]
SHORT Explanation for this: mech related to above ACE AND ACE Inhibitors and BP dynamcs. Standby for full expl......
http://www.physorg.com/print136426165.html Angiotensin receptor blockers are lower incidence, progression of Alzheimer's disease July 28th, 2008 in Medicine & Health / Diseases Researchers at Boston University School of Medicine (BUSM) have, for the first time, found that angiotensin receptor blockers (ARBs)—a particular class of anti-hypertensive medicines—are associated with a striking decrease in the occurrence and progression of dementia. Data from this study will be presented this weekend (July 27) at the 2008 International Conference on Alzheimer's disease in Chicago. Using data from the Decision Support System Database of the U.S. Department of Health System Veterans Affairs (with information on more than 5 million people), researchers looked at records from patients using ARBs, and compared them with subjects who had a similar health status, but were taking different medications. They found patients taking ARBs had about a 35-40 percent lower chance of getting Alzheimer's disease or dementia. The researchers also examined patients who were already suffering from Alzheimer's disease or dementia, and found those subjects had up to a 45 percent lower chance of developing delirium, being admitted to nursing homes or dying. Patients who appeared to benefit particularly well from use of ARBs were those who had experienced strokes before or during the course of their illness. According to the researchers these results suggest that ARBs might protect against developing Alzheimer's disease and dementia. "For those who already have dementia, use of ARBs might delay deterioration of brain function and help keep patients out of nursing homes," said lead presenter Benjamin Wolozin, MD, PhD, a professor of pharmacology at BUSM. "The study is particularly interesting because we compared the effects of ARBs to other medications used for treating blood pressure or cardiovascular disease. This suggests that ARBs are more effective than other blood pressure and cardiovascular medications for preventing Alzheimer's disease or dementia," he added. Although the researchers are unsure why ARBs might be so beneficial, they believe one possibility suggested by prior studies on animal models is that ARBs help prevent nerve cell injury from blood vessel damage or help promote nerve cell recovery after blood vessel damage. Damage to blood vessels is thought to reduce brain capacity and promote dementia, so reducing this damage might prevent the occurrence or progression of dementia. Source: Boston University
ACE-I vs angiotensin II receptor antagonists vs Vasopeptidase inhibitor (VPI)[edit]
Insulin: Heart Function & Inflammation[edit]
Unicode In typesetting technical literature β.Unicode number for β is U+03B2, and with β or β the β is coded in HTML.[edit]
In typesetting technical literature, it is a commonly made mistake to use the German letter ß as a replacement for the β. The two letters resemble each other superficially, but they are unrelated. This substitution looks extremely unprofessional to the eyes of German or Greek readers. The Unicode number for β is U+03B2, and with β or β the β is coded in HTML. The internal version, ϐ, is encoded as U+03D0 in Unicode or ϐ in HTML. S
References[edit]
- ^ Hartmann T, Bieger SC, Brühl B; et al. (1997). "Distinct sites of intracellular production for Alzheimer's disease Aβ40/42 amyloid peptides". Nat. Med. 3 (9): 1016–20. doi:10.1038/nm0997-1016. PMID 9288729.
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: Explicit use of et al. in:|author=
(help)CS1 maint: multiple names: authors list (link) - ^ Yin YI, Bassit B, Zhu L, Yang X, Wang C, Li YM (2007). "γ-Secretase Substrate Concentration Modulates the Aβ42/Aβ40 Ratio: Implications for Alzheimer's disease". J. Biol. Chem. 282 (32): 23639–44. doi:10.1074/jbc.M704601200. PMID 17556361.
{{cite journal}}
: CS1 maint: multiple names: authors list (link) CS1 maint: unflagged free DOI (link) - ^ FitzGerald RJ, Murray BA, Walsh DJ. Hypotensive peptides from milk proteins. J Nutr 2004;134:980S-8S. PMID 15051858.
- ^ Aihara K, Kajimoto O, Hirata H, Takahashi R, Nakamura Y. Effect of powdered fermented milk with Lactobacillus helveticus on subjects with high-normal blood pressure or mild hypertension. J Am Coll Nutr. 2005 Aug;24(4):257-65 PMID 16093403.
- ^ Dive, V.; et al. (2004), "Review: Phosphinic peptides as zinc metalloproteinase inhibitors.", Cellular and Molecular Life Science, 61: 2010–2019
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: Explicit use of et al. in:|author=
(help) Phosphinic peptides as zinc metalloproteinase inhibitors - ^ Gerogiadis, D.; Guniasse, P.; Cotton, J.; Yiotakis, A.; Dive, V. (2004), "Structural Determinants of RXPA380, a Potent and Highly Selective Inhibitor of the Angiotensin-Converting Enzyme C-domain.", Biochemistry, 43: 8048–8054, doi:10.1021/bi049504q