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Serum components, enzymes, peptides, polysaccharides, RNA-types, viruses, lipids and ribosomes have been separated using Diethylaminoethyl cellulose.

Diethylaminoethyl-cellulose, a substituted cellulose derivative used in bead form for chromatography of acidic or slightly basic proteins at pH values above their isoelectric point.

The Sepharose types are particularly useful for the separation of high molecular weight proteins.

Ion-exchange media, usually have the ions bound to the charged groups on the resin are called ‘counter ions’. For CM-cellulose the counter ion is usually Na+ and for DEAE-cellulose the counter ion is normally Cl¯.

The DEAE-cellulose pH range is 2-9; the ion capacity is .11 - .16 mmol (Cl-)/ml[1]

  1. ^ Himmelhoch, SR (1971). Chromatography of proteins on ion-exchange adsorbents. Meth. Enzymol. 22. pp. 276–286.