Wikipedia talk:USEP/Courses/JHU MolBio Ogg SP14/Group 82B

Hi Randel what are your thoughts on aminoallyl nucleotides as a article of choice? MishaMishasubz (talk) 09:27, 2 March 2014 (UTC)

I think this would be a good article to pick as there is not much information yet, there is a lot of detail we can add to better the quality of this article. I feel this information will be helpful to those looking for non-radioactive forms of labeling DNA. Mishasubz (talk) 22:22, 2 March 2014 (UTC)

This would be a good article to work on. I've already have some links posted to help us get started, though we probably need more in depth information about aminoallyl nucleotides. Would you agree that we need to identify functionality (binding mechanism), florescence information, and other purposes? What other ideas do you have for headings/subheadings we could look into?Crandel5425 (talk) 04:01, 4 March 2014 (UTC)

a little confused about the separate section for capping enzyme? should that not be under functionality/MOA (mechanism of action)why is that a separate section?

I agree with your headings/subheadings, would like to add maybe, structure features, if any that separate the different amino acids.Along with images if we can find any. A section mentioning short-comings or complications involved such as high background?? what do you think. Also we should probably give a general introduction on aminoallyl and lead into aminoallyl modified nucleotides.Mishasubz (talk) 07:59, 4 March 2014 (UTC)

The capping enzyme was based on the previous assignment to pick 2 articles and input what was needed in them. Good ideas, the intro is a great segway into our topic. — Preceding unsigned comment added by Crandel5425 (talk • contribs) 01:48, 5 March 2014 (UTC)

Unit 7- article prep work

hi Randel, I am having a difficult time finding information on nucleotides other than dUTP, do you have any suggestions? Mishasubz (talk) 07:57, 10 March 2014 (UTC)

WP:MOSINTRO Aminoallyl lead section practice Mishasubz (talk) 08:25, 10 March 2014 (UTC)

Unit 8 progress report

• updated the reference list with other articles
• added information on biotin-labeling
• removed and modified sections

Mishasubz (talk) 21:06, 22 March 2014 (UTC)

Unit 10 - Progress report

See Project page.

final revisions

Hi Randel, In the process of revision, I have a few specific questions;

"The DNA or RNA from a sample is copied in vitro by T7 polymerase, RT polymerase or some other polymerase (except of eukaryotic origin) in a mix of nucleotides in which one, typically uracil (RNA and also DNA), is a mix aminoallyl-NTP and normal UTP. An alternative method is using thiol reactive dyes". I am little confused about this sentence, can we explain it further, it seems to be missing a beginning and end; this is a process of labeling? right? does it go along with the Example protocol by Holly Bennet and Joe DeRisi originated at Rosetta Informatics modified by Chris Seidel. [11]Mishasubz (talk) 09:02, 25 April 2014 (UTC)

Fix as needed, I think it got lost in moving things around. Crandel5425 (talk) 13:35, 25 April 2014 (UTC)

Final progress report

MOVED TO Project page

Progress Report

• Follow martinhyou's review and respond to his comments
• add in mapping image — Preceding unsigned comment added by Crandel5425 (talk • contribs) 04:07, 1 May 2014 (UTC)

So I have fixed up the 'use' section as per Martins review. I am not sure what we are doing about the citations from 6-11, do you know what needs to be fixed? thanksMishasubz (talk) 05:54, 1 May 2014 (UTC)

I think you fixed them as they don't show red anymore. Crandel5425 (talk) 18:41, 1 May 2014 (UTC)