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Hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) is a version of partition chromatography and occupies the opposite end of the partition spectrum from reversed phase liquid chromatography. HILIC Partition Method Graphic
Both are distinct from normal phase liquid chromatography in that water is part of the mobile phase. The name was suggested by Dr. Andrew Alpert in his 1990 paper on the subject.[1]. He described the chromatographic mechanism for it as liquid-liquid partition chromatography where analytes elute in order of increasing polarity.

Surface[edit]

Any polar chromatographic surface can be used for HILIC separations. Even non-polar bonded silicas have been used with extremely high organic solvent composition, when the silica used for the chromatographic media was particularly polar. With that exception, HILIC phases can be grouped into five categories of neutral polar or ionic surfaces:

Heckendorf (talk) 20:42, 25 May 2016 (UTC)==Mobile phase== A typical mobile phase for HILIC chromatography includes acetonitrile ("MeCN", also designated as "ACN") with a small amount of water. However, any aprotic solvent miscible with water (e.g. THF or dioxane) can be used. Alcohols can also be used, however, their concentration must be higher to achieve the same degree of retention for an analyte relative to an aprotic solvent - water combination. See also Aqueous Normal Phase Chromatography

It is commonly believed that in HILIC, the mobile phase forms a water-rich layer on the surface of the polar stationary phase vs. the water-deficient mobile phase, creating a liquid/liquid extraction system. The analyte is distributed between these two layers[2][3][4]. However, HILIC is more than just simple partitioning and includes hydrogen donor interactions between neutral polar species as well as weak electrostatic mechanisms under the high organic solvent conditions used for retention. This distinguishes HILIC as a mechanism distinct from ion exchange chromatography. The more polar compounds will have a stronger interaction with the stationary aqueous layer than the less polar compounds. Thus, a separation based on a compound's polarity and degree of solvation takes place.

Additives[edit]

Ionic additives, such as ammonium acetate and ammonium formate, are usually used to control the mobile phase pH and ion strength. In HILIC they can also contribute to the polarity of the analyte, resulting in differential changes in retention. For extremely polar analytes (e.g. aminoglycoside antibiotics (gentamicin) or Adenosine triphosphate), higher concentrations of buffer (ca. 100mM) are required to assure that the analyte will be in a single ionic form. Otherwise asymmetric peak shape, chromatographic tailing, and/or poor recovery from the stationary phase will be observed. For the separation of neutral polar analytes (e.g. carbohydrates), no buffer is necessary.

Use of other salts such as 100-300mM sodium perchlorate, which are soluble in high-organic solvent mixtures (ca. 70%-90% acetonitrile), can be used to increase the mobile phase polarity to effect elution. These salts are not volatile, so this technique is less useful with a mass spectrometer as the detector. Usually a gradient (to increasing amounts of water) is enough to promote elution.

All ions partition into the stationary phase to some degree, so an occasional "wash" with water is required to ensure a reproducible stationary phase.

Uses[edit]

The HILIC mode of separation is used extensively for separation of some biomolecules, organic and some inorganic molecules[5] by differences in polarity. Its utility has increased due to the simplified sample preparation for biological samples, when analyzing for metabolites, since the metabolic process generally results in the addition of polar groups to enhance elimination from the cellular tissue. For the detection of polar compounds with the use of electrospray-ionization mass spectrometry as a chromatographic detector, HILIC can offer a tenfold increase in sensitivity over reversed-phase chromatography[5] because the organic solvent is much more volatile.

Choice of pH[edit]

With surface chemistries that are weakly ionic, the choice of pH can affect the ionic nature of the column chemistry. Properly adjusted, the pH can be set to reduce the selectivity toward functional groups with the same charge as the column, or enhance it for oppositely charged functional groups. Similarly, the choice of pH affects the polarity of the solutes. However, for column surface chemistries that are strongly ionic, and thus resistant to pH values in the mid-range of the pH scale (pH 3.5-8.5), these separations will be reflective of the polarity of the analytes alone, and thus might be easier to understand when doing methods development.

ERLIC[edit]

In 2008, Alpert coined the term, ERLIC[6] (Electrostatic Repulsion Hydrophilic Interaction Chromatography), for HILIC separations where an ionic column surface chemistry is used to repel a common ionic polar group on an analyte or within a set of analytes, to facilitate separation by the remaining polar groups. Electrostatic effects have an order of magnitude stronger chemical potential than neutral polar effects. This allows one to minimize the influence of a common, ionic group within a set of analyte molecules; or to reduce the degree of retention from these more polar functional groups, even enabling isocratic separations in lieu of a gradient in some situations. His subsequent publication further described Orientation Effects[7] which others have also called Ion-Pair Normal Phase[8] or e-HILIC, reflecting retention mechanisms sensitive to a particular ionic portion of the analyte, either attractive or repulsive. ERLIC (eHILIC) separations need not be isocratic, but the net effect is the reduction of the attraction of a particularly strong polar group, which then requires less strong elution conditions, and the enhanced interaction of the remaining polar functional groups in the analyte.

Cationic eHILIC[edit]

For example, one could use a cation exchange (negatively charged) surface chemistry for ERLIC separations to reduce the influence on retention of anionic (negatively charged) groups (the phosphates of nucleotides or of phosphonyl antibiotic mixtures; or sialic acid groups of modified carbohydrates) to now allow separation based more on the basic and/or neutral functional groups of these molecules. Modifying the polarity of a weakly ionic group (e.g. carboxyl) on the surface is easily accomplished by adjusting the pH to be within two pH units of that group's pKa. For strongly ionic functional groups of the surface (i.e. sulfates or phosphates) one could instead use a lower amount of buffer so the residual charge is not completely ion paired. An example of this would be the use of a 15mM (rather than the recommended >20mM buffer), pH 9.2 mobile phase on a polymeric, zwitterionic, betaine-sulfonate surface to separate phosphonyl antibiotic mixtures (each containing a phosphate group). This enhances the influence of the column's sulfonic acid functional groups of its surface chemistry over its, slightly diminished (by pH), quaternary amine. Commensurate with this, these analytes will show a reduced retention on the column eluting earlier, and in higher amounts of organic solvent, than if a neutral polar HILIC surface were used. This also increases their detection sensitivity by negative ion mass spectrometry.

Anionic eHILIC[edit]

By analogy to the above, one can use an anion exchange (positively charged) column surface chemistry to reduce the influence on retention of cationic (positively charged) functional groups for a set of analytes, such as when selectively isolating phosphorylated peptides or sulfated polysaccharide molecules. Use of a pH between 1 and 2 pH units will reduce the polarity of two of the three ionizable oxygens of the phosphate group, and thus will allow easy desorption from the (oppositely charged) surface chemistry. It will also reduce the influence of negatively charged carboxyls in the analytes, since they will be protonated at this low a pH value, and thus contribute less overall polarity to the molecule. Any common, positively charged amino groups will be repelled from the column surface chemistry and thus these conditions enhance the role of the phosphate's polarity (as well as other neutral polar groups) in the separation.

References[edit]

  1. ^ Alpert, Andrew J. (1990). "Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compounds". Journal of Chromatography. 499: 177–196. doi:10.1016/S0021-9673(00)96972-3. PMID 2324207.
  2. ^ Verhaar, L.A.Th.; Kuster, B.F.M. (1982). "Contribution to the elucidation of the mechanism of sugar retention on amine-modified silica in liquid chromatography". J. Chromatogr. A. 234: 57–64. doi:10.1016/S0021-9673(00)81780-X.
  3. ^ Nikolov, Zivko L.; Reilly, Peter J. (1985). "Retention of carbohydrates on silica and amine-bonded silica stationary phases: application of the hydration model". J. Chromatogr. A. 325: 287–293. doi:10.1016/S0021-9673(00)96030-8.
  4. ^ Ortho, P.; Engelhardt, H. (1982). "Separation of sugars on chemically modified silica gel". Chromatographia. 15: 91–96.
  5. ^ a b Eric S. Grumbach; et al. (October 2004). "Hydrophilic Interaction Chromatography Using Silica Columns for the Retention of Polar Analytes and Enhanced ESI-MS Sensitivity". LCGC Magazine. Retrieved 2008-07-14. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: date and year (link)
  6. ^ Alpert, Andrew J. (January 2008). "Electrostatic Repulsion Hydrophilic Interaction Chromatography for Isocratic Separation of Charged Solutes and Selective Isolation of Phosphopeptides". Anal. Chem. 80 (1): 62–76. doi:10.1021/ac070997p. PMID 18027909.{{cite journal}}: CS1 maint: date and year (link)
  7. ^ Alpert, Andrew J.; et al. (June 2010). "Peptide Orientation Affects Selectivity in Ion-Exchange Chromatography". Anal. Chem. 82 (12): 5253–5259. doi:10.1021/ac100651k. PMC 2884984. PMID 20481592. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: date and year (link)
  8. ^ Ding, W.; et al. (September 2009). "Identification and Quantification of Glycoproteins Using Ion-Pairing Normal-Phase LC and MS". Molecular & Cellular Proteomics. 8 (9): 2170–2185. doi:10.1074/mcp.M900088-MCP200. PMC 2742440. PMID 19525481. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: date and year (link) CS1 maint: unflagged free DOI (link)

Category:Chromatography Category:Laboratory techniques Category:Molecular biology Category:Biochemistry methods

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