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SIL1

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SIL1
Identifiers
AliasesSIL1, BAP, MSS, ULG5, SIL1 nucleotide exchange factor
External IDsOMIM: 608005; MGI: 1932040; HomoloGene: 32544; GeneCards: SIL1; OMA:SIL1 - orthologs
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001037633
NM_022464

NM_030749
NM_001360814

RefSeq (protein)

NP_001032722
NP_071909

NP_109674
NP_001347743

Location (UCSC)Chr 5: 138.95 – 139.29 MbChr 18: 35.4 – 35.63 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Nucleotide exchange factor SIL1 is a protein that in humans is encoded by the SIL1 gene.[5][6][7][8]

This gene encodes a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in this gene have been associated with Marinesco-Sjogren syndrome. Alternate transcriptional splice variants have been characterized.[8]

Discovery and expression

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In the mid-1990s, several laboratories independently discovered Sil1 in three different organisms. A conditional lethality screen was used in the yeast Yarrowia lipolytica to detect cellular components that interacted with the 7S RNA or the signal recognition particle (SRP) during co-translational translocation, which led to the identification of the Sls1p gene. Sls1p is an ER-luminal, 54 kDa protein that contains an N-terminal signal sequence and a C-terminal ER-retention motif.[9] Sls1p fractionated with the membranous fraction and was shown to interact with the Sec61p translocation apparatus. Sls1p was induced by ER stresses, such as heat shock or inhibition of glycosylation, and its deletion resulted in decreased maturation of secretory proteins. This protein also bound to the ADP-bound form of Kar2p, the yeast homolog of BiP, and stimulated its interaction with Sec63, a DnaJ family member and a translocon component. Disruption of the interaction between Sls1p and Kar2p significantly affected the secretion of a reporter protein, leading the investigators to conclude that Sls1p assisted BiP in the translocation of nascent proteins into the ER lumen. Another group working with Saccharomyces cerevisiae conducted a screen for genes that would suppress the severe growth defect observed in the ΔIre1ΔLhs1 yeast double mutants when overexpressed and named this gene the suppressor of Ire1 and Lhs1 deletion 1 (Sil1). Sil1p was shown to be a homolog of Sls1p, and a combined deletion of Sil1p and Lhs1 (yeast GRP170) proved to be lethal after causing a total block in protein translocation into the ER. Mammalian (human) SIL1 was discovered in a yeast two-hybrid screen aimed at identifying proteins that interacted with a mutant ATPase domain of BiP and was initially named BiP-associated protein (BAP). Sequence analysis revealed that BAP is a mammalian homolog of Sls1p and Sil1p and is similar to the cytosolic HSP70-binding protein, HSPBP1. Biochemical data further demonstrated that BAP also had NEF activity for BiP.

Structure and mechanism of nucleotide exchange

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Structural data for yeast Sil1p revealed an elongated, “kidney bean”-like molecular shape that consists of 16 α-helices (A1–A16) and lacks β-sheets. The central helices A3-A14 form the armadillo (ARM)-like repeats (ARM1-ARM4), which are named after those found in β-catenin. Each ARM repeat is composed of three α-helices that pack into a superhelix. A crystal structure of yeast Sil1p complexed with the ADP-bound NBD of Kar2p/BiP revealed that the ARM domain of Sil1p wraps around lobe IIb of BiP’s NBD and makes additional contacts with lobe Ib. This interaction causes lobes Ib and IIb to rotate away from each other, leading to ADP release. Point mutations in the Sil1p-interacting site of Kar2p’s NBD specifically disrupted Sil1p binding but retained Kar2p’s ability to interact with Lhs1/GRP170, the other ER NEF, providing the first indication that their mechanisms of NEF activity were different. Homology mapping and comparisons with the structure of cytosolic HSPBP1 indicated that the region of SIL1 encoded by exons 6 and 9 constitutes major BiP-binding sites, and exon 10 encodes a minor interaction site.

Tissue expression

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Although SIL1 is ubiquitously expressed, levels vary widely by tissue. The Human Protein Atlas (www.proteinatlas.org) shows that the expression pattern of SIL1 mirrors that of BiP even more closely than that of GRP170, which is a known UPR target, as is BiP. Perhaps the different expression pattern for GRP170 is due to its dual function as a chaperone and a NEF for BiP. SIL1 expression at a single-cell level has also been determined in a number of tissues, which does not strictly correlate with the secretory capacity of the cell, and tissues displaying the highest relative levels of SIL1 are not necessarily those most affected by loss of its function. This might imply an additional function, which has been demonstrated for Sil1p, or an as yet undiscovered role of SIL1 in certain tissues. Understanding this reason for variation in tissue expression will require further studies.

SIL1
Identifiers
AliasesSIL1, BAP, MSS, ULG5, SIL1 nucleotide exchange factor
External IDsOMIM: 608005; MGI: 1932040; HomoloGene: 32544; GeneCards: SIL1; OMA:SIL1 - orthologs
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001037633
NM_022464

NM_030749
NM_001360814

RefSeq (protein)

NP_001032722
NP_071909

NP_109674
NP_001347743

Location (UCSC)Chr 5: 138.95 – 139.29 MbChr 18: 35.4 – 35.63 Mb
PubMed search[10][11]
Wikidata
View/Edit HumanView/Edit Mouse

Interactions

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SIL1 has been shown to interact with Binding immunoglobulin protein.[6]

References

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  1. ^ a b c d e f GRCh38: Ensembl release 89: ENSG00000120725Ensembl, May 2017
  2. ^ a b c d e f GRCm38: Ensembl release 89: ENSMUSG00000024357Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Tyson JR, Stirling CJ (December 2000). "LHS1 and SIL1 provide a lumenal function that is essential for protein translocation into the endoplasmic reticulum". The EMBO Journal. 19 (23): 6440–52. doi:10.1093/emboj/19.23.6440. PMC 305876. PMID 11101517.
  6. ^ a b Chung KT, Shen Y, Hendershot LM (December 2002). "BAP, a mammalian BiP-associated protein, is a nucleotide exchange factor that regulates the ATPase activity of BiP". The Journal of Biological Chemistry. 277 (49): 47557–63. doi:10.1074/jbc.M208377200. PMID 12356756.
  7. ^ Senderek J, Krieger M, Stendel C, Bergmann C, Moser M, Breitbach-Faller N, et al. (December 2005). "Mutations in SIL1 cause Marinesco-Sjögren syndrome, a cerebellar ataxia with cataract and myopathy". Nature Genetics. 37 (12): 1312–4. doi:10.1038/ng1678. PMID 16282977. S2CID 28860307.
  8. ^ a b "Entrez Gene: SIL1 SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae)".
  9. ^ Ichhaporia VP, Hendershot LM (February 2021). "Role of the HSP70 Co-Chaperone SIL1 in Health and Disease". International Journal of Molecular Sciences. 22 (4): 1564. doi:10.3390/ijms22041564. PMC 7913895. PMID 33557244.
  10. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  11. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

Further reading

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